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SH3BP5L Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81381

Novus Biologicals, part of Bio-Techne
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NBP1-81381
NBP1-81381-25ul

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: CQQAEARVQALQKTLRRAIGKSRPYFELKAQFSQILEEHKAKVTELEQQVAQAKTRYSVALRNLEQISEQIHARRRGGLPPHPLGPRRSSPVGAEAGPED

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SH3BP5L Antibody

Western Blot: SH3BP5L Antibody [NBP1-81381]

Western Blot: SH3BP5L Antibody [NBP1-81381]

Western Blot: SH3BP5L Antibody [NBP1-81381] - Analysis in control (vector only transfected HEK293T lysate) and SH3BP5L over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: SH3BP5L Antibody [NBP1-81381]

Immunocytochemistry/ Immunofluorescence: SH3BP5L Antibody [NBP1-81381]

Immunocytochemistry/Immunofluorescence: SH3BP5L Antibody [NBP1-81381] - Staining of human cell line A-431 shows localization to nucleoplasm, the Golgi apparatus & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SH3BP5L Antibody [NBP1-81381]

Immunohistochemistry-Paraffin: SH3BP5L Antibody [NBP1-81381]

Immunohistochemistry-Paraffin: SH3BP5L Antibody [NBP1-81381] - Staining of human duodenum shows strong membranous positivity in glandular cells.

Applications for SH3BP5L Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SH3BP5L

Alternate Names

FLJ33845, KIAA1720SH3BP-5-like, SH3 domain-binding protein 5-like, SH3-binding domain protein 5-like

Gene Symbol

SH3BP5L

Additional SH3BP5L Products

Product Documents for SH3BP5L Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SH3BP5L Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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