SHANK1 Antibody (N22/21)

Western Blot: SHANK1 Antibody (N22/21) [NBP2-12915]

Western Blot: SHANK1 Antibody (N22/21) [NBP2-12915] - Western Blot analysis of Rat brain membrane lysate showing detection of SHANK1 protein using Mouse Anti-SHANK1 Monoclonal Antibody, Clone S22-21 (NBP2-12915). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK1 Monoclonal Antibody (NBP2-12915) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody (N22/21) [NBP2-12915]

Immunocytochemistry/Immunofluorescence: SHANK1 Antibody (N22/21) [NBP2-12915] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK1 Monoclonal Antibody, Clone S22-21 (NBP2-12915). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SHANK1 Monoclonal Antibody (NBP2-12915) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SHANK1 Antibody (D) Composite.

Immunohistochemistry: SHANK1 Antibody (N22/21) [NBP2-12915]

Immunohistochemistry: SHANK1 Antibody (N22/21) [NBP2-12915] - Immunohistochemistry analysis using Mouse Anti-SHANK1 Monoclonal Antibody, Clone S22-21 (NBP2-12915). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-SHANK1 Monoclonal Antibody (NBP2-12915) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Filaggrin-like staining (upper layer aggregations of staining).