Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Microarray, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # N23b/49

Concentration

1 mg/ml

View all SHANK2 Antibodies »

Product Specifications

Immunogen

Fusion protein amino acids 84-309 of rat Shank2

Localization

Cytoplasm , Cell Junction , Synapse , Postsynaptic Cell Membrane , Postsynaptic Density , Cell Projection , Growth Cone

Specificity

Detects approx 160kDa. Recognizes Shank1, 2 and 3.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for SHANK2 Antibody (N23b/49)

Western Blot: SHANK2 Antibody (N23b/49) [NBP2-12913]

Western Blot: SHANK2 Antibody (N23b/49) [NBP2-12913]

Western Blot: SHANK2 Antibody (N23b/49) [NBP2-12913] - Western Blot analysis of Rat brain membrane lysate showing detection of SHANK2 protein using Mouse Anti-SHANK2 Monoclonal Antibody, Clone S23b-49 (NBP2-12913). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK2 Monoclonal Antibody (NBP2-12913) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Immunocytochemistry/ Immunofluorescence: SHANK2 Antibody (N23b/49) [NBP2-12913]

Immunocytochemistry/ Immunofluorescence: SHANK2 Antibody (N23b/49) [NBP2-12913]

Immunocytochemistry/Immunofluorescence: SHANK2 Antibody (N23b/49) [NBP2-12913] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK2 (pan) Monoclonal Antibody, Clone S23b-49 (NBP2-12913). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SHANK2 (pan) Monoclonal Antibody (NBP2-12913) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SHANK2 (pan) Antibody (D) Composite.
Immunocytochemistry/ Immunofluorescence: SHANK2 Antibody (N23b/49) [NBP2-12913]

Immunocytochemistry/ Immunofluorescence: SHANK2 Antibody (N23b/49) [NBP2-12913]

Immunocytochemistry/Immunofluorescence: SHANK2 Antibody (N23b/49) [NBP2-12913] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK2 (pan) Monoclonal Antibody, Clone S23b-49 (NBP2-12913). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SHANK2 (pan) Monoclonal Antibody (NBP2-12913) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) SHANK2 (pan) Antibody. (D) Composite.

Applications for SHANK2 Antibody (N23b/49)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:1000

Immunohistochemistry-Paraffin

0.1-1.0 ug/ml

Western Blot

1:1000
Application Notes
1 ug/ml of SHANK (pan) Antibody was sufficient for detection of Shank1-4 in 10 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SHANK2

1 ug/mL was sufficient for detection of Shank1-4 in 10ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody.

Long Name

SH3 and Multiple Ankyrin Repeat Domains Protein 2

Alternate Names

AUTS17, CORTBP1, CTTNBP1, ProSAP1, SPANK-3

Entrez Gene IDs

22941 (Human); 210274 (Mouse); 171093 (Rat)

Gene Symbol

SHANK2

UniProt

Q9QX74 (Rat)

Additional SHANK2 Products

Product Documents for SHANK2 Antibody (N23b/49)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SHANK2 Antibody (N23b/49)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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