Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Microarray, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # S69

Concentration

1 mg/ml

View all SHANK3 Antibodies »

Product Specifications

Immunogen

Synthetic peptide amino acids 840-857 of rat Shank3

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Localization

Cytoplasm , Cell Junction , Synapse , Postsynaptic Cell Membrane , Postsynaptic Density

Specificity

Detects approx 190kDa. No cross-reactivity against Shank1 or Shank2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for SHANK3 Antibody (S69)

Western Blot: SHANK3 Antibody (S69) [NBP1-47610]

Western Blot: SHANK3 Antibody (S69) [NBP1-47610]

Western Blot: SHANK3 Antibody (S69) [NBP1-47610] - Western Blot analysis of Rat brain membrane lysate showing detection of SHANK3 protein using Mouse Anti-SHANK3 Monoclonal Antibody, Clone S69-46 (NBP1-47610). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK3 Monoclonal Antibody (NBP1-47610) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Immunocytochemistry/ Immunofluorescence: SHANK3 Antibody (S69) [NBP1-47610]

Immunocytochemistry/ Immunofluorescence: SHANK3 Antibody (S69) [NBP1-47610]

Immunocytochemistry/Immunofluorescence: SHANK3 Antibody (S69) [NBP1-47610] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK3 Monoclonal Antibody, Clone S69-46 (NBP1-47610). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SHANK3 Monoclonal Antibody (NBP1-47610) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SHANK3 Antibody (D) Composite.
Immunohistochemistry: SHANK3 Antibody (S69) [NBP1-47610]

Immunohistochemistry: SHANK3 Antibody (S69) [NBP1-47610]

Immunohistochemistry: SHANK3 Antibody (S69) [NBP1-47610] - Immunohistochemistry analysis using Mouse Anti-SHANK3 Monoclonal Antibody, Clone S69-46 (NBP1-47610). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-SHANK3 Monoclonal Antibody (NBP1-47610) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Early stages of filaggrin-like and dermal staining.
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Applications for SHANK3 Antibody (S69)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100

Western Blot

1:1000
Application Notes
1 ug/ml of SHANK3 Antibody was sufficient for detection of Shank3 in 10 ug COS cell lysate transiently transfected with Shank3 by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary Antibody.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SHANK3

Function: Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and Homer, respectively, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction.; Subcellular location: Cytoplasm. Cell junction synapse. Cell junction synapse postsynaptic cell membrane postsynaptic density. Note: Postsynaptic density of neuronal cells. Extends into the region subjacent to the PSD.; Tissue specificity: Widely expressed in brain.

Alternate Names

DEL22q13.3, KIAA1650proline rich synapse associated protein 2, Proline-rich synapse-associated protein 2, PROSAP2, PSAP2SH3 and multiple ankyrin repeat domains protein 3, SH3 and multiple ankyrin repeat domains 3, shank postsynaptic density protein, Shank3, SPANK-2

Entrez Gene IDs

59312 (Rat)

Gene Symbol

SHANK3

UniProt

Q9JLU4 (Rat)

Additional SHANK3 Products

Product Documents for SHANK3 Antibody (S69)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SHANK3 Antibody (S69)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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