Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Microarray, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # S69

Format

BSA Free

Concentration

1 mg/ml

View all SHANK3 Antibodies »

Product Specifications

Immunogen

Synthetic peptide amino acids 840-857 of rat Shank3

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Localization

Cytoplasm , Cell Junction , Synapse , Postsynaptic Cell Membrane , Postsynaptic Density

Specificity

Detects approx 190kDa. No cross-reactivity against Shank1 or Shank2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for SHANK3 Antibody (S69) - BSA Free

Western Blot: SHANK3 Antibody (S69) [NBP1-47610]

Western Blot: SHANK3 Antibody (S69) [NBP1-47610]

Western Blot: SHANK3 Antibody (S69) [NBP1-47610] - Western Blot analysis of Rat brain membrane lysate showing detection of SHANK3 protein using Mouse Anti-SHANK3 Monoclonal Antibody, Clone S69-46 (NBP1-47610). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK3 Monoclonal Antibody (NBP1-47610) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Immunocytochemistry/ Immunofluorescence: SHANK3 Antibody (S69) [NBP1-47610]

Immunocytochemistry/ Immunofluorescence: SHANK3 Antibody (S69) [NBP1-47610]

Immunocytochemistry/Immunofluorescence: SHANK3 Antibody (S69) [NBP1-47610] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK3 Monoclonal Antibody, Clone S69-46 (NBP1-47610). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SHANK3 Monoclonal Antibody (NBP1-47610) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SHANK3 Antibody (D) Composite.
Immunohistochemistry: SHANK3 Antibody (S69) [NBP1-47610]

Immunohistochemistry: SHANK3 Antibody (S69) [NBP1-47610]

Immunohistochemistry: SHANK3 Antibody (S69) [NBP1-47610] - Immunohistochemistry analysis using Mouse Anti-SHANK3 Monoclonal Antibody, Clone S69-46 (NBP1-47610). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-SHANK3 Monoclonal Antibody (NBP1-47610) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Early stages of filaggrin-like and dermal staining.
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Applications for SHANK3 Antibody (S69) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100

Western Blot

1:1000
Application Notes
1 ug/ml of SHANK3 Antibody was sufficient for detection of Shank3 in 10 ug COS cell lysate transiently transfected with Shank3 by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary Antibody.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SHANK3

Function: Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and Homer, respectively, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction.; Subcellular location: Cytoplasm. Cell junction synapse. Cell junction synapse postsynaptic cell membrane postsynaptic density. Note: Postsynaptic density of neuronal cells. Extends into the region subjacent to the PSD.; Tissue specificity: Widely expressed in brain.

Alternate Names

DEL22q13.3, KIAA1650proline rich synapse associated protein 2, Proline-rich synapse-associated protein 2, PROSAP2, PSAP2SH3 and multiple ankyrin repeat domains protein 3, SH3 and multiple ankyrin repeat domains 3, shank postsynaptic density protein, Shank3, SPANK-2

Entrez Gene IDs

59312 (Rat)

Gene Symbol

SHANK3

UniProt

Q9JLU4 (Rat)

Additional SHANK3 Products

Product Documents for SHANK3 Antibody (S69) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SHANK3 Antibody (S69) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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