Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all SHANK3 Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 1475 to 1525 of human SH3 and Multiple Ankyrin Repeat Domains 3 using the numbering given in entry Q9BYB0 (GeneID 85358).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SHANK3 Antibody

Western Blot: SHANK3 Antibody [NBP2-32161]

Western Blot: SHANK3 Antibody [NBP2-32161]

Western Blot: SHANK3 Antibody [NBP2-32161] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-SHANK3 antibody NBP2-32161 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: SHANK3 Antibody [NBP2-32161]

Immunoprecipitation: SHANK3 Antibody [NBP2-32161]

Immunoprecipitation: SHANK3 Antibody [NBP2-32161] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SHANK3 antibody NBP2-32161 used for IP at 6 ug/mg lysate. For blotting immunoprecipitated SHANK3, NBP2-32161 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for SHANK3 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000 - 1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SHANK3

The SHANK3 gene encodes a SH3 and multiple ankyrin repeat domain protein 3 that in isoform 1 is 1,741 amino acids long at 186 kDA and in isoform 2 is 1,732 amino acids long at 185 kDA. This protein functions as an adaptor in postsynaptic density of excitatory synapses. Through complexes that include GKAP/PSD-95 and Homer and the actin-based cytoskeleton, these excitatory synapses are connected with the postsynaptic membrane including NMDA-type and metabotropic glutamate receptor. Additionally, this protein may function in structural and organizational projects of the dendritic spine and the synaptic junction. SHANK3 gene participates in cytoplasmic microtubules, glutamatergic synapses, and signaling events regulated by ret tyrosine kinase. It is known to interact with genes PLCG1, FYN, SRC, ABL1, and PIK3R1. Defects in SHANK3 are associated with autism spectrum disorders and schizophrenia type 15. It is also associated with phelan-mcdermid syndrome, cerebritis, prostatitis, neuronitis, dementia, prostate cancer, hypogonadism, hepatitis a, jacobsen syndrome, and trisomy 22.

Alternate Names

DEL22q13.3, KIAA1650proline rich synapse associated protein 2, Proline-rich synapse-associated protein 2, PROSAP2, PSAP2SH3 and multiple ankyrin repeat domains protein 3, SH3 and multiple ankyrin repeat domains 3, shank postsynaptic density protein, Shank3, SPANK-2

Gene Symbol

SHANK3

UniProt

Q9BYB0 (Human)

Additional SHANK3 Products

Product Documents for SHANK3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SHANK3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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