SHC1 Antibody (OTI3A1) [CoraFluor™ 1]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-74167CL1

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Unconjugated

NBP2-02434

Alexa Fluor 350

NBP2-74167AF350

Alexa Fluor 405

NBP2-74167AF405

Alexa Fluor 488

NBP2-74167AF488

Alexa Fluor 532

NBP2-74167AF532

Alexa Fluor 594

NBP2-74167AF594

Alexa Fluor 647

NBP2-74167AF647

Alexa Fluor 700

NBP2-74167AF700

Alexa Fluor 750

NBP2-74167AF750

Biotin

NBP2-74167B

DyLight 350

NBP2-74167UV

DyLight 405

NBP2-74167V

DyLight 488

NBP2-74167G

DyLight 550

NBP2-74167R

DyLight 594

NBP2-74167DL594

DyLight 650

NBP2-74167C

DyLight 680

NBP2-74167FR

DyLight 755

NBP2-74167IR

FITC

NBP2-74167F

HRP

NBP2-74167H

Janelia Fluor 525

NBP2-74167JF525

Janelia Fluor 549

NBP2-74167JF549

Janelia Fluor 585

NBP2-74167JF585

Janelia Fluor 635

NBP2-74167JF635

Janelia Fluor 646

NBP2-74167JF646

Janelia Fluor 669

NBP2-74167JF669

Forumulation

Catalog #

Azide and BSA Free

NBP2-74167

Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

CoraFluor 1

Antibody Source

Monoclonal Mouse IgG₁ Clone # OTI3A1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SHC1 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human SHC1 (NP_003020) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for SHC1 Antibody (OTI3A1) [CoraFluor™ 1]

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: SHC1

Signaling adapter that couples activated growth factor receptors to signaling pathway. Isoform p46Shc andisoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of theGRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoformp52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shcdoes not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular responseto oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and isindispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome crelease and apoptosis. The expression of isoform p66Shc has been correlated with life span

Long Name

SHC-transforming Protein 1

Alternate Names

SHCA

Gene Symbol

SHC1

Additional SHC1 Products

Product Documents for SHC1 Antibody (OTI3A1) [CoraFluor™ 1]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for SHC1 Antibody (OTI3A1) [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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