SHP-1 Antibody (SR41-02)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67321

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-67321
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR41-02

Concentration

1 mg/ml

View all SHP-1 Antibodies »

Product Summary for SHP-1 Antibody (SR41-02)

Immunogen

Synthetic peptide within Human SHP-1 aa 537-595 / 595. (SwissProt: P29350 Human; SwissProt: P29351 Mouse; SwissProt: P81718 Rat)

Localization

Cytoplasm, Nucleus.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SHP-1 Antibody (SR41-02)

Western Blot: SHP-1 Antibody (SR41-02) [NBP2-67321]

Western Blot: SHP-1 Antibody (SR41-02) [NBP2-67321]

Western Blot: SHP-1 Antibody (SR41-02) [NBP2-67321] - Analysis of SHP1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Raji cell lysate Lane 2: Jurkat cell lysate
Immunocytochemistry/ Immunofluorescence: SHP-1 Antibody (SR41-02) [NBP2-67321]

Immunocytochemistry/ Immunofluorescence: SHP-1 Antibody (SR41-02) [NBP2-67321]

Immunocytochemistry/Immunofluorescence: SHP-1 Antibody (SR41-02) [NBP2-67321] - Staining SHP1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: SHP-1 Antibody (SR41-02) [NBP2-67321]

Immunohistochemistry-Paraffin: SHP-1 Antibody (SR41-02) [NBP2-67321]

Immunohistochemistry-Paraffin: SHP-1 Antibody (SR41-02) [NBP2-67321] - Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-SHP-1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 m
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Applications for SHP-1 Antibody (SR41-02)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:1000
Application Notes
IP assay-dependent
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SHP-1

The SH2 domain-containing phosphatases, SHP-1 and SHP-2, are well-studied cytosolic tyrosine phosphatases that share many structural and regulatory features. They both have two tandem SH2 domains at the N-terminus, followed by a catalytic domain, and an inhibitory C-terminus. Despite their structural similarity, the enzymes appear to have different physiological roles and exhibit different expression patterns. SHP-1 is highly expressed in hematopoietic tissues, and in general has a negative impact on lymphocyte signaling. In contrast, SHP-2 is widely expressed, and in general promotes signaling pathways that lead to differentiation, cell growth, or migration.

Long Name

Src Homology Region 2 Domain Phosphatase 1

Alternate Names

HCPH, PTP1C, PTPN6, SHP1

Gene Symbol

PTPN6

Additional SHP-1 Products

Product Documents for SHP-1 Antibody (SR41-02)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SHP-1 Antibody (SR41-02)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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