SHP-2/PTPN11 [p Tyr542] Antibody (2E2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26397

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2E2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SHP-2 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human SHP-2/PTPN11 [p Tyr542] [UniProt Q06124]

Modification

p Tyr 542

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SHP-2/PTPN11 [p Tyr542] Antibody (2E2)

SHP-2/PTPN11 [p Tyr542] Antibody (2E2)

Immunoprecipitation: SHP-2/PTPN11 [p Tyr542] Antibody (2E2) [NBP3-26397] -

Immunoprecipitation: SHP-2/PTPN11 [p Tyr542] Antibody (2E2) [NBP3-26397] - Immunoprecipitating SHP-2/PTPN11 [p Tyr542] in Hela whole cell lysate treated with Pervanadate.
Lane 1: Rabbit control IgG (1ug) instead of NBP3-26397 in Hela whole cell lysate treated with Pervanadate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).
Lane 2: NBP3-26397 (3ug) + Hela whole cell lysate treated with Pervanadate (1mg).
Lane 3: Hela whole cell lysate treated with Pervanadate (20ug).
SHP-2/PTPN11 [p Tyr542] Antibody (2E2)

Western Blot: SHP-2/PTPN11 [p Tyr542] Antibody (2E2) [NBP3-26397] -

Western Blot: SHP-2/PTPN11 [p Tyr542] Antibody (2E2) [NBP3-26397] - Positive Western Blot detected in Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate (treated with Pervanadate or not).
All lanes: SHP-2/PTPN11 [p Tyr542] Antibody at 0.65ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 68 KDa
Observed band size: 68 KDa

Applications for SHP-2/PTPN11 [p Tyr542] Antibody (2E2)

Application
Recommended Usage

Immunoprecipitation

1:200-1:1000

Western Blot

1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: SHP-2

SHP-2 (also called PTP2C, SH-PTP2, PTP1D, SH-PTP3, Syp and PTPN11) is a protein tyrosine phosphatase (PTP) expressed in most cell types (unlike SHP-1), (1). SHP-2 is involved in t the signal transduction stimulated by EGF, PDGF and Insulin. After cell stimulation, SHP-2 (as well as SHP-1), translocates from cytosol to the plasma membrane and binds to tyrosine-phosphorylated receptors through their SH2 domains, becoming activated in the process (2). When phosphorylated, SHP-2 has been shown to bind Grb2, EGFR, PDGFR, IRS-1 via its SH2 domain (3). Over expression of SHP-2 leads to Juvenile Myelo-monocytic Leukemia and Noonan syndrome (4-5).

Long Name

Src Homology Region 2 Domain Phosphatase 2

Alternate Names

PTP1D, PTP2C, PTPN11, SH-PTP2, SHP2

Gene Symbol

PTPN11

Additional SHP-2 Products

Product Documents for SHP-2/PTPN11 [p Tyr542] Antibody (2E2)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SHP-2/PTPN11 [p Tyr542] Antibody (2E2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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