SHP-2/PTPN11 Antibody (6G11)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26357

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6G11

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SHP-2 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human SHP-2/PTPN11 [UniProt Q06124]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SHP-2/PTPN11 Antibody (6G11)

SHP-2/PTPN11 Antibody (6G11)

Immunohistochemistry: SHP-2/PTPN11 Antibody (6G11) [NBP3-26357] -

Immunohistochemistry: SHP-2/PTPN11 Antibody (6G11) [NBP3-26357] - Image of SHP-2/PTPN11 Antibody (6G11) diluted at 1:100 and staining in paraffin-embedded human heart tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
SHP-2/PTPN11 Antibody (6G11)

Western Blot: SHP-2/PTPN11 Antibody (6G11) [NBP3-26357] -

Western Blot: SHP-2/PTPN11 Antibody (6G11) [NBP3-26357] - Positive Western Blot detected in: Jurkat whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, K562 whole cell lysate, HL60 whole cell lysate.
All lanes: SHP-2/PTPN11 Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 69, 53 kDa
Observed band size: 69 kDa

Applications for SHP-2/PTPN11 Antibody (6G11)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: SHP-2

SHP-2 (also called PTP2C, SH-PTP2, PTP1D, SH-PTP3, Syp and PTPN11) is a protein tyrosine phosphatase (PTP) expressed in most cell types (unlike SHP-1), (1). SHP-2 is involved in t the signal transduction stimulated by EGF, PDGF and Insulin. After cell stimulation, SHP-2 (as well as SHP-1), translocates from cytosol to the plasma membrane and binds to tyrosine-phosphorylated receptors through their SH2 domains, becoming activated in the process (2). When phosphorylated, SHP-2 has been shown to bind Grb2, EGFR, PDGFR, IRS-1 via its SH2 domain (3). Over expression of SHP-2 leads to Juvenile Myelo-monocytic Leukemia and Noonan syndrome (4-5).

Long Name

Src Homology Region 2 Domain Phosphatase 2

Alternate Names

PTP1D, PTP2C, PTPN11, SH-PTP2, SHP2

Gene Symbol

PTPN11

Additional SHP-2 Products

Product Documents for SHP-2/PTPN11 Antibody (6G11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SHP-2/PTPN11 Antibody (6G11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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