SHP-2/PTPN11 Antibody (M163)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23400

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # M163

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SHP-2 Antibodies »

Product Specifications

Immunogen

Clone (M163) was generated from a recombinant protein containing amino acids in the N-terminal region of human SHP-2/PTPN11. This sequence is highly conserved in rat and mouse SHP-2/PTPN11. [UniProt# Q06124]

Specificity

The antibody detects a 72 kDa protein in human A431 and Jurkat cells, and mouse brain. This antibody does not cross-react with SHP1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

72 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for SHP-2/PTPN11 Antibody (M163)

SHP-2/PTPN11 Antibody (M163)

Western Blot: SHP-2/PTPN11 Antibody (M163) [NBP3-23400] -

Western Blot: SHP-2/PTPN11 Antibody (M163) [NBP3-23400] - Western blot analysis of adult mouse brain. The blot was probed with anti-SHP2 (N-terminal) antibody at 1:250 (lane 1), 1:500 (lane 2), 1:1000 (lane 3), and 1:2000 (lane 4).

Applications for SHP-2/PTPN11 Antibody (M163)

Application
Recommended Usage

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: SHP-2

SHP-2 (also called PTP2C, SH-PTP2, PTP1D, SH-PTP3, Syp and PTPN11) is a protein tyrosine phosphatase (PTP) expressed in most cell types (unlike SHP-1), (1). SHP-2 is involved in t the signal transduction stimulated by EGF, PDGF and Insulin. After cell stimulation, SHP-2 (as well as SHP-1), translocates from cytosol to the plasma membrane and binds to tyrosine-phosphorylated receptors through their SH2 domains, becoming activated in the process (2). When phosphorylated, SHP-2 has been shown to bind Grb2, EGFR, PDGFR, IRS-1 via its SH2 domain (3). Over expression of SHP-2 leads to Juvenile Myelo-monocytic Leukemia and Noonan syndrome (4-5).

Long Name

Src Homology Region 2 Domain Phosphatase 2

Alternate Names

PTP1D, PTP2C, PTPN11, SH-PTP2, SHP2

Gene Symbol

PTPN11

Additional SHP-2 Products

Product Documents for SHP-2/PTPN11 Antibody (M163)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SHP-2/PTPN11 Antibody (M163)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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