SHP-2/PTPN11 [p Tyr542] Antibody (SR2126)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21539

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-21539-50ul
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR2126

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SHP-2 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human phospho-SHP-2/PTPN11 [Tyr542] (Uniprot #: Q06124)

Modification

p Tyr 542

Specificity

Detects endogenous levels of phospho-SHP-2/PTPN11 [Tyr542]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SHP-2/PTPN11 [p Tyr542] Antibody (SR2126)

Western Blot: SHP-2/PTPN11 [p Tyr 542] Antibody (SR2126) [NBP3-21539] -

Western Blot: SHP-2/PTPN11 [p Tyr 542] Antibody (SR2126) [NBP3-21539] - Western blot analysis using NBP3-21539 in (1) NIH/3T3 cell lysates; (2) NIH/3T3 cell lysates treated with PDGF.

Applications for SHP-2/PTPN11 [p Tyr542] Antibody (SR2126)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunoprecipitation

1:50

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SHP-2

SHP-2 (also called PTP2C, SH-PTP2, PTP1D, SH-PTP3, Syp and PTPN11) is a protein tyrosine phosphatase (PTP) expressed in most cell types (unlike SHP-1), (1). SHP-2 is involved in t the signal transduction stimulated by EGF, PDGF and Insulin. After cell stimulation, SHP-2 (as well as SHP-1), translocates from cytosol to the plasma membrane and binds to tyrosine-phosphorylated receptors through their SH2 domains, becoming activated in the process (2). When phosphorylated, SHP-2 has been shown to bind Grb2, EGFR, PDGFR, IRS-1 via its SH2 domain (3). Over expression of SHP-2 leads to Juvenile Myelo-monocytic Leukemia and Noonan syndrome (4-5).

Long Name

Src Homology Region 2 Domain Phosphatase 2

Alternate Names

PTP1D, PTP2C, PTPN11, SH-PTP2, SHP2

Gene Symbol

PTPN11

Additional SHP-2 Products

Product Documents for SHP-2/PTPN11 [p Tyr542] Antibody (SR2126)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SHP-2/PTPN11 [p Tyr542] Antibody (SR2126)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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