SHP-2/PTPN11 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-806
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Bovine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-YENVGLMQQQKSFR corresponding to C-Terminus according to NP_002825.3.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for SHP-2/PTPN11 Antibody
Western Blot: SHP-2/PTPN11 Antibody [NB100-806]
Western Blot: SHP-2/PTPN11 Antibody [NB100-806] - Human Muscle lysate (RIPA buffer, 35 ug total protein per lane). Antibody at 2 ug/mL. Detected by chemiluminescence.Immunocytochemistry/ Immunofluorescence: SHP-2/PTPN11 Antibody [NB100-806]
Immunocytochemistry/Immunofluorescence: SHP-2/PTPN11 Antibody [NB100-806] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).Flow Cytometry: SHP-2/PTPN11 Antibody [NB100-806]
Flow Cytometry: SHP-2/PTPN11 Antibody [NB100-806] - Analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.Applications for SHP-2/PTPN11 Antibody
Application
Recommended Usage
Flow Cytometry
10 ug/mL
Immunocytochemistry/ Immunofluorescence
10 ug/mL
Peptide ELISA
Detection limit 1:28000
Western Blot
1 - 3 ug/mL
Application Notes
WB: Approx. 70 kDa band observed in human skeletal muscle lysate (calculated MW of 68.0 kDa band according to NP_002825.3).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: SHP-2
Long Name
Src Homology Region 2 Domain Phosphatase 2
Alternate Names
PTP1D, PTP2C, PTPN11, SH-PTP2, SHP2
Gene Symbol
PTPN11
UniProt
Additional SHP-2 Products
Product Documents for SHP-2/PTPN11 Antibody
Product Specific Notices for SHP-2/PTPN11 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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