SIAH1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20356
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Predicted:
Bovine (100%), Chimpanzee (100%), Rat (99%), Rhesus Macaque (100%), Xenopus (99%), Zebrafish (97%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Proximity Ligation Assay, Western Blot
Cited:
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human SIAH1. The exact sequence is proprietary.
Localization
Cytoplasm; Nucleus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for SIAH1 Antibody
![Western Blot: SIAH1 Antibody [NBP2-20356]](https://resources.bio-techne.com/images/products/SIAH1-Antibody-Western-Blot-NBP2-20356-img0006.jpg "Western Blot: SIAH1 Antibody [NBP2-20356]")
Western Blot: SIAH1 Antibody [NBP2-20356]
Western Blot: SIAH1 Antibody [NBP2-20356] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with SIAH1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.![Immunohistochemistry-Paraffin: SIAH1 Antibody [NBP2-20356]](https://resources.bio-techne.com/images/products/SIAH1-Antibody-Immunohistochemistry-Paraffin-NBP2-20356-img0004.jpg "Immunohistochemistry-Paraffin: SIAH1 Antibody [NBP2-20356]")
Immunohistochemistry-Paraffin: SIAH1 Antibody [NBP2-20356]
Immunohistochemistry-Paraffin: SIAH1 Antibody [NBP2-20356] - Human lung Papillary adenocarcinoma, using SIAH1 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.Applications for SIAH1 Antibody
Application
Recommended Usage
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Immunoprecipitation
Reported in scientific literature (PMID: 25587023)
Proximity Ligation Assay
Assay dependent
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: SIAH1
Alternate Names
E3 ubiquitin-protein ligase SIAH1, EC 6.3.2, EC 6.3.2.-, FLJ08065, hSIAH1, HUMSIAH, Seven in absentia homolog 1, seven in absentia homolog 1 (Drosophila), Siah-1, SIAH1A, Siah-1a
Gene Symbol
SIAH1
UniProt
Additional SIAH1 Products
Product Documents for SIAH1 Antibody
Product Specific Notices for SIAH1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.