Siglec-1/CD169 Antibody (HSn 7D2) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB600-534

Novus Biologicals, part of Bio-Techne
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NB600-534
NB600-534SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Porcine, Virus

Cited:

Human, Mouse, Porcine, Virus

Applications

Validated:

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, In vitro assay, In vivo assay, Radioimmunoassay, Western Blot

Cited:

Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, In vitro assay, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # HSn 7D2

Format

BSA Free

Concentration

1.0 mg/ml

View all Siglec-1/CD169 Antibodies »

Product Specifications

Immunogen

Full-length purified human Sialoadhesin (d1-4)Fc protein. [Swiss-Prot# Q9BZZ2]

Epitope

Within domains 1-4.

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 27441395). Mouse reactivity reported in scientific literature. Not yet tested in other species. Use in Virus reported in scientific literature (PMID:27487279).

Localization

Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Siglec-1/CD169 Antibody (HSn 7D2) - BSA Free

Immunohistochemistry-Frozen: Siglec-1/CD169 Antibody (HSn 7D2) - BSA Free [NB600-534]

Immunohistochemistry-Frozen: Siglec-1/CD169 Antibody (HSn 7D2) - BSA Free [NB600-534]

Immunohistochemistry-Frozen: Siglec-1/CD169 Antibody (HSn 7D2) [NB600-534] - Adult mouse small intestine. NB600-534 Siglec-1 was used at 1:500 and labeled with Alexa Fluor 568 conjugated secondary antibody (Red). DAPI shown as blue. Image from verified customer review.
Flow Cytometry: Siglec-1/CD169 Antibody (HSn 7D2) - BSA Free [NB600-534]

Flow Cytometry: Siglec-1/CD169 Antibody (HSn 7D2) - BSA Free [NB600-534]

Flow Cytometry: Siglec-1/CD169 Antibody (HSn 7D2) [NB600-534] - Human CD14+ PBMC differentiated to M1 macrophages with rhGM-CSF were stained with Mouse Anti-Siglec-1/CD169 Monoclonal Antibody (NB600-534, filled histogram), or Mouse IgG1 isotype control (MAB002, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (F0101B).
Immunohistochemistry-Paraffin: Siglec-1/CD169 Antibody (HSn 7D2) - BSA Free [NB600-534]

Immunohistochemistry-Paraffin: Siglec-1/CD169 Antibody (HSn 7D2) - BSA Free [NB600-534]

Immunohistochemistry-Paraffin: Siglec-1/CD169 Antibody (HSn 7D2) [NB600-534] - Staining of Sialoadhesin in human spleen using DAB with hematoxylin counterstain.

Applications for Siglec-1/CD169 Antibody (HSn 7D2) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:10-1:1000

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 28794041)

Immunohistochemistry

1:10-1:500. Use reported in scientific literature (PMID 26106049)

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

In vivo assay

reported in scientific literature (PMID 18414664)

Western Blot

reported in scientific literature
Application Notes
This Sialoadhesin Antibody (HSn 7D2) is useful for Immunohistochemistry (paraffin-embedded, acetone-fixed, and frozen sections), Flow Cytometry, Radioimmunoassay and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NB600-534 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Siglec-1/CD169

Sialoadhesin, also known as sialic acid-binding Ig-like lectin 1, siglec-1, CD169, SIGLEC1, and SN, is a member of the sialic acid binding Ig-like lectin (SIGLEC) family. The SIGLECs are a family of membrane bound lectins that belong to the immunoglobulin superfamily. SIGLECs bind sialic acid and mediate cell to cell interactions. Family members include sialoadhesin, CD22 and CD33. Sialoadhesin is a lectin-like macrophage-specific cell adhesion molecule that mediates sialic acid dependent binding of glycoconjugate ligands on activated macrophages, lymphocytes, granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. The Sialoadhesin protein is found in two locations within the cell, the cell membrane (isoform 1) and secreted in the cytoplasm (isoform 2). Sialoadhesin is expressed in spleen, lymph node, brain, bone marrow, liver, colon and lung tissues and in inflammatory macrophages in rheumatoid arthritis.

Long Name

Sialic Acid Binding Ig-like Lectin 1

Alternate Names

CD169, Siglec1

Entrez Gene IDs

6614 (Human)

Gene Symbol

SIGLEC1

UniProt

Q9BZZ2 (Human)

Additional Siglec-1/CD169 Products

Product Documents for Siglec-1/CD169 Antibody (HSn 7D2) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Siglec-1/CD169 Antibody (HSn 7D2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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