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Siglec-1/CD169 Antibody (HSn 7D2) [Janelia Fluor® 549]

Novus Biologicals, part of Bio-Techne | Catalog # NB600-534JF549

Novus Biologicals, part of Bio-Techne
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NB600-534JF549

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Porcine, Virus

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, In vitro assay, In vivo assay, Radioimmunoassay, Western Blot

Label

Janelia Fluor 549

Antibody Source

Monoclonal Mouse IgG1 Clone # HSn 7D2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for Siglec-1/CD169 Antibody (HSn 7D2) [Janelia Fluor® 549]

Immunogen

Full-length purified human Sialoadhesin (d1-4)Fc protein. [Swiss-Prot# Q9BZZ2]

Epitope

Within domains 1-4.

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 27441395). Mouse reactivity reported in scientific literature. Not yet tested in other species. Use in Virus reported in scientific literature (PMID:27487279).

Localization

Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Siglec-1/CD169 Antibody (HSn 7D2) [Janelia Fluor® 549]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

In vitro assay

Optimal dilutions of this antibody should be experimentally determined.

In vivo assay

Optimal dilutions of this antibody should be experimentally determined.

Radioimmunoassay

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Siglec-1/CD169

The siglecs are a family of membrane bound lectins (of the immunoglobulin superfamily) that bind sialic acid and mediate cell-cell interactions. Family members include sialoadhesin, CD22 and CD33. Sialoadhesin is a macrophage-specific cell adhesion molecule that is expressed on activated macrophages in chronic inflammation and in tumours.

Long Name

Sialic Acid Binding Ig-like Lectin 1

Alternate Names

CD169, Siglec1

Gene Symbol

SIGLEC1

Additional Siglec-1/CD169 Products

Product Documents for Siglec-1/CD169 Antibody (HSn 7D2) [Janelia Fluor® 549]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Siglec-1/CD169 Antibody (HSn 7D2) [Janelia Fluor® 549]

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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