Siglec-2/CD22 Antibody (2H1C4) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37327

Novus Biologicals, part of Bio-Techne
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NBP2-37327
NBP2-37327-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2H1C4

Format

BSA Free

Concentration

1 mg/ml

View all Siglec-2/CD22 Antibodies »

Product Summary for Siglec-2/CD22 Antibody (2H1C4) - BSA Free

Immunogen

Purified recombinant fragment of human Siglec-2/CD22 (AA: 621-725) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

95.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Siglec-2/CD22 Antibody (2H1C4) - BSA Free

Western Blot: Siglec-2/CD22 Antibody (2H1C4)BSA Free [NBP2-37327]

Western Blot: Siglec-2/CD22 Antibody (2H1C4)BSA Free [NBP2-37327]

Western Blot: Siglec-2/CD22 Antibody (2H1C4) [NBP2-37327] - Analysis using CD22 mouse mAb against L1210 (1), Hela (2), HEK293 (3), Jurkat (4), OCM-1 (5), A432 (6) and NIH/3T3 (7) cell lysate.
Immunocytochemistry/ Immunofluorescence: Siglec-2/CD22 Antibody (2H1C4) - BSA Free [NBP2-37327]

Immunocytochemistry/ Immunofluorescence: Siglec-2/CD22 Antibody (2H1C4) - BSA Free [NBP2-37327]

Immunocytochemistry/Immunofluorescence: Siglec-2/CD22 Antibody (2H1C4) [NBP2-37327] - Immunofluorescence analysis of Hela cells using CD22 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody (2H1C4) - BSA Free [NBP2-37327]

Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody (2H1C4) - BSA Free [NBP2-37327]

Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody (2H1C4) [NBP2-37327] - Analysis of cerebellum tissues using CD22 mouse mAb with DAB staining.
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Applications for Siglec-2/CD22 Antibody (2H1C4) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Siglec-2/CD22

CD22 is a single-pass type 1 membrane glycoprotein found in the cytoplasm and cell membrane of B-lymphocytes. CD22 has at least two isoforms (alpha and beta) produced by alternate splicing. The protein exists predominantly as a monomer of the beta isoform (95 kDa). It is also found as a heterodimer of the beta and alpha isoforms.

Long Name

Sialic Acid Binding Ig-like Lectin 2

Alternate Names

BL-CAM, CD22, Siglec2

Gene Symbol

CD22

Additional Siglec-2/CD22 Products

Product Documents for Siglec-2/CD22 Antibody (2H1C4) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Siglec-2/CD22 Antibody (2H1C4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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