Key Product Details

Species Reactivity

Human

Applications

CyTOF-reported, Flow Cytometry

Label

CoraFluor 1

Antibody Source

Monoclonal Mouse IgG1 Clone # 194211

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Siglec-7/CD328 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Siglec-7
Gln19-Gly357 (predicted)
Accession # Q9Y286

Specificity

Detects human Siglec-7 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Siglec-6, -8, -9 or recombinant Siglec-E is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for Siglec-7/CD328 Antibody (194211) [CoraFluor™ 1]

Application
Recommended Usage

CyTOF-reported

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: Siglec-7/CD328

Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds toalpha-2,3- and alpha-2,6-linked sialic acid. Also binds disialogangliosides (disialogalactosyl globoside, disialyllactotetraosylceramide and disialyl GalNAc lactotetraoslylceramide). The sialic acid recognition site may be masked bycis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptorupon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) thatblock signal transduction through dephosphorylation of signaling molecules. Mediates inhibition of natural killercells cytotoxicity. May play a role in hemopoiesis. Inhibits differentiation of CD34+ cell precursors towardsmyelomonocytic cell lineage and proliferation of leukemic myeloid cells (in vitro)

Long Name

Sialic Acid Binding Ig-like Lectin 7

Alternate Names

AIRM1, CD328, QA79, Siglec7

Gene Symbol

SIGLEC7

Additional Siglec-7/CD328 Products

Product Documents for Siglec-7/CD328 Antibody (194211) [CoraFluor™ 1]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Siglec-7/CD328 Antibody (194211) [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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