Key Product Details

Species Reactivity

Human, Canine, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1D9

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Siglec-9 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human SIGLEC9 (NP_055256) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

50.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Siglec-9 Antibody (OTI1D9) - Azide and BSA Free

Western Blot: Siglec-9 Antibody (OTI1D9)Azide and BSA Free [NBP2-74189]

Western Blot: Siglec-9 Antibody (OTI1D9)Azide and BSA Free [NBP2-74189]

Western Blot: Siglec-9 Antibody (OTI1D9) - Azide and BSA Free [NBP2-74189] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Siglec-9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Siglec-9.
Immunohistochemistry: Siglec-9 Antibody (OTI1D9) - Azide and BSA Free [NBP2-74189]

Immunohistochemistry: Siglec-9 Antibody (OTI1D9) - Azide and BSA Free [NBP2-74189]

Immunohistochemistry: Siglec-9 Antibody (OTI1D9) - Azide and BSA Free [NBP2-74189] - Staining of paraffin-embedded Human liver tissue using anti-Siglec-9 mouse monoclonal antibody.
Western Blot: Siglec-9 Antibody (OTI1D9)Azide and BSA Free [NBP2-74189]

Western Blot: Siglec-9 Antibody (OTI1D9)Azide and BSA Free [NBP2-74189]

Western Blot: Siglec-9 Antibody (OTI1D9) - Azide and BSA Free [NBP2-74189] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Siglec-9 monoclonal antibody.
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Applications for Siglec-9 Antibody (OTI1D9) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50

Western Blot

1:2000-40000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Siglec-9

Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- or alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface

Long Name

Sialic Acid Binding Ig-like Lectin 9

Alternate Names

CD329, OBBP-Like, Siglec9

Gene Symbol

SIGLEC9

Additional Siglec-9 Products

Product Documents for Siglec-9 Antibody (OTI1D9) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Siglec-9 Antibody (OTI1D9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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