Key Product Details

Species Reactivity

Validated:

Mouse

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

PE (Excitation = 488 nm, Emission = 575 nm)

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # 23M14A10

Concentration

1.0 mg/ml

View all Siglec-H Antibodies »

Product Summary for Siglec-H Antibody (23M14A10) [PE]

Immunogen

SkRSHTkRVERPATHMDQA (aa286-304)

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Applications for Siglec-H Antibody (23M14A10) [PE]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Siglec-H

Siglec-H, or sialic acid binding immunoglobulin-like lectin H, is a CD33 related protein expressed specifically by plasmacytoid dendritic cells or pDCs (1, 2). Antigen-mediated delivery by Siglec-H in pDCs inhibits Th cell proliferation and further antibody responsesThis leads to lessened expansion and Th1/Th17 polarization (3). Constant and low density antigen presentation by Siglec-H is thought to lead to an exhaustive type lessening of the response in CD4+ cells but not tolerance. A number of pathways have been proposed for the Siglec-H induced T cell hyporesponsiveness. Ever decreasing activation cycles in the presence of low level but continuous antigen delivery, as that observed with Siglec-H, have also been demonstrated to impart FoxP3+ Tregs immunosuppressive tolerogen-like effects (4, 5). Ability to identify and control Siglec-H antigen mediated delivery activities with specific antibody provides a focal point for potential development of inflammation controls.

Long Name

Sialic Acid Binding Ig-like Lectin 7

Alternate Names

SiglecH

Gene Symbol

Siglech

Additional Siglec-H Products

Product Documents for Siglec-H Antibody (23M14A10) [PE]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Siglec-H Antibody (23M14A10) [PE]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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