SIL1 Antibody (OTI3E3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-02377

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3E3

Concentration

0.56 mg/ml

View all SIL1 Antibodies »

Product Summary for SIL1 Antibody (OTI3E3)

Immunogen

Full length human recombinant protein of human SIL1 (NP_071909) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

52.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SIL1 Antibody (OTI3E3)

Western Blot: SIL1 Antibody (OTI3E3) [NBP2-02377]

Western Blot: SIL1 Antibody (OTI3E3) [NBP2-02377]

Western Blot: SIL1 Antibody (3E3) [NBP2-02377] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIL1.
Immunocytochemistry/Immunofluorescence: SIL1 Antibody (3E3) [NBP2-02377] Staining of COS7 cells transiently transfected by pCMV6-ENTRY SIL1.
Immunohistochemistry-Paraffin: SIL1 Antibody (OTI3E3) [NBP2-02377]

Immunohistochemistry-Paraffin: SIL1 Antibody (OTI3E3) [NBP2-02377]

Immunohistochemistry-Paraffin: SIL1 Antibody (3E3) [NBP2-02377] - Staining of paraffin-embedded liver tissue using anti-SIL1 mouse monoclonal antibody.
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Applications for SIL1 Antibody (OTI3E3)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:100-500

Immunohistochemistry-Paraffin

1:50

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.56 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SIL1

This gene encodes a resident endoplasmic reticulum (ER), N-linked glycoprotein with an N-terminal ER targeting sequence, 2 putative N-glycosylation sites, and a C-terminal ER retention signal. This protein functions as a nucleotide exchange factor for another unfolded protein response protein. Mutations in this gene have been associated with Marinesco-Sjogren syndrome. Alternate transcriptional splice variants have been characterized.

Alternate Names

BAPMarinesco-Sjogren syndrome, BiP-associated protein, MSS, nucleotide exchange factor SIL1, SIL1 homolog, endoplasmic reticulum chaperone (S. cerevisiae), ULG5

Entrez Gene IDs

64374 (Human)

Gene Symbol

SIL1

Additional SIL1 Products

Product Documents for SIL1 Antibody (OTI3E3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SIL1 Antibody (OTI3E3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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