SIRP alpha/CD172a Antibody (BLR049F)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14675

Recombinant Monoclonal
Novus Biologicals, part of Bio-Techne
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NBP3-14675
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit Clone # BLR049F

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SIRP alpha/CD172a Antibodies »

Product Summary for SIRP alpha/CD172a Antibody (BLR049F)

Immunogen

residues 31-368 (ECD)

Clonality

Monoclonal

Host

Rabbit

Scientific Data Images for SIRP alpha/CD172a Antibody (BLR049F)

Western Blot: SIRP alpha/CD172a Antibody (BLR049F) [NBP3-14675]

Western Blot: SIRP alpha/CD172a Antibody (BLR049F) [NBP3-14675]

Western Blot: SIRP alpha/CD172a Antibody [NBP3-14675] - Whole cell lysate (50 ug) from Malme-3M, HeLa, 786-O, HEK293T, U2OS, OVCAR-4, and SK-MEL-28 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SIRP alpha recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit antiActin.
Immunocytochemistry/ Immunofluorescence: SIRP alpha/CD172a Antibody (BLR049F) [NBP3-14675]

Immunocytochemistry/ Immunofluorescence: SIRP alpha/CD172a Antibody (BLR049F) [NBP3-14675]

Immunocytochemistry/Immunofluorescence: SIRP alpha/CD172a Antibody [NBP3-14675] - Malme-3M cells. Antibody: Rabbit anti-SIRP alpha recombinant monoclonal antibody used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Immunohistochemistry-Paraffin: SIRP alpha/CD172a Antibody (BLR049F) [NBP3-14675]

Immunohistochemistry-Paraffin: SIRP alpha/CD172a Antibody (BLR049F) [NBP3-14675]

Immunohistochemistry-Paraffin: SIRP alpha/CD172a Antibody [NBP3-14675] - Human breast carcinoma. Antibody: Rabbit anti-SIRP alpha recombinant monoclonalantibody [BLR049F] used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG.Substrate: DAB.
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Applications for SIRP alpha/CD172a Antibody (BLR049F)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 - 1:500

Immunohistochemistry

1:100- 1:500

Immunohistochemistry-Paraffin

1:100- 1:500

Immunoprecipitation

20 ul/1 mg lysate

Western Blot

1:1000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

>95%

Formulation

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: SIRP alpha/CD172a

Protein tyrosine phosphatases (PTPases) SHP-1 and SHP-2 are critical regulators in the intracellular signaling pathways that result in cell responses such as mitosis, differentiation, migration, survival, transformation or death. SHP-2 is a signal transducer for several receptor tyrosine kinases and cytokine receptors. A novel SHP-2 associated glycoprotein was recently cloned from human, rat, mouse and cattle by several labs and was designated SIRPa (1), SHPS-1 (2,3), MyD-1 (4), BIT (5,6) and p84 (7). SIRPa is a new gene family containing at least fifteen members. SIRPa is a substrate of many activated tyrosine kinases such as insulin receptor, EGFR, PDGFR and src, and a specific docking protein for SHP-2 (1,2,5,8). SIRPa has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion and plays a general role in different physiological and pathological processes (1,2,5,8).

Long Name

Signal-regulatory Protein alpha

Alternate Names

BIT, CD172a, MFR, MYD-1, SHPS1, SIRPA

Gene Symbol

SIRPA

Additional SIRP alpha/CD172a Products

Product Documents for SIRP alpha/CD172a Antibody (BLR049F)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SIRP alpha/CD172a Antibody (BLR049F)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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