Sirtuin 1/SIRT1 Antibody (PCRP-SIRT1-1E11)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13916

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # PCRP-SIRT1-1E11

Concentration

0.2 mg/ml

View all Sirtuin 1/SIRT1 Antibodies »

Product Summary for Sirtuin 1/SIRT1 Antibody (PCRP-SIRT1-1E11)

Immunogen

Recombinant full-length human Sirtuin 1/SIRT1protein (Uniprot: Q96EB6)

Localization

Cytoplasm, Mitochondrion and Nucleus, PML body

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14290)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Sirtuin 1/SIRT1 Antibody (PCRP-SIRT1-1E11)

Immunocytochemistry/ Immunofluorescence: Sirtuin 1/SIRT1 Antibody (PCRP-SIRT1-1E11) [NBP3-13916]

Immunocytochemistry/ Immunofluorescence: Sirtuin 1/SIRT1 Antibody (PCRP-SIRT1-1E11) [NBP3-13916]

Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody (PCRP-SIRT1-1E11) [NBP3-13916] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using Sirtuin 1/SIRT1 Antibody (PCRP-SIRT1-1E11) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
Protein Array: Sirtuin 1/SIRT1 Antibody (PCRP-SIRT1-1E11) [NBP3-13916]

Protein Array: Sirtuin 1/SIRT1 Antibody (PCRP-SIRT1-1E11) [NBP3-13916]

Protein Array: Sirtuin 1/SIRT1 Antibody (PCRP-SIRT1-1E11) [NBP3-13916] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Sirtuin 1/SIRT1 Antibody (PCRP-SIRT1-1E11).

Applications for Sirtuin 1/SIRT1 Antibody (PCRP-SIRT1-1E11)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunoprecipitation

1-2 ug/100-500 ug protein

Western Blot

1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Sirtuin 1/SIRT1

The S. cerevisiae silent information regulator 2 (Sir2) protein is an NAD+-dependent histone deacetylase (HDACs) that plays a critical role in transcriptional silencing, genome stability, and longevity (1-2). Part of the Sirtuin family (Sir2, Sir3, Sir3), yeast Sir2 is the founding member of Class III HDACs. A human homologue of Sir2 , SIRT1, regulates the activity of the p53 tumor suppressor and inhibits apoptosis (3-4).

Long Name

Silent Mating Type Information Regulation 2 Homolog

Alternate Names

SIR2-like protein 1, SIR2alpha, SIR2L1, SIRT1

Gene Symbol

SIRT1

UniProt

Q96EB6 (Human)

Additional Sirtuin 1/SIRT1 Products

Product Documents for Sirtuin 1/SIRT1 Antibody (PCRP-SIRT1-1E11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Sirtuin 1/SIRT1 Antibody (PCRP-SIRT1-1E11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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