Sirtuin 1/SIRT1 Antibody (PCRP-SIRT1-1E11) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14290
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Protein Array, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # PCRP-SIRT1-1E11
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for Sirtuin 1/SIRT1 Antibody (PCRP-SIRT1-1E11) - Azide and BSA Free
Immunogen
Recombinant full-length human Sirtuin 1/SIRT1protein (Uniprot: Q96EB6)
Localization
Cytoplasm, Mitochondrion and Nucleus, PML body
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13916).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for Sirtuin 1/SIRT1 Antibody (PCRP-SIRT1-1E11) - Azide and BSA Free
Protein Array: Sirtuin 1/SIRT1 Antibody (PCRP-SIRT1-1E11) - Azide and BSA Free [NBP3-14290]
Protein Array: Sirtuin 1/SIRT1 Antibody (PCRP-SIRT1-1E11) - Azide and BSA Free [NBP3-14290] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Sirtuin 1/SIRT1 Antibody (PCRP-SIRT1-1E11).Applications for Sirtuin 1/SIRT1 Antibody (PCRP-SIRT1-1E11) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunoprecipitation
1-2 ug per 100-500 ug protein
Western Blot
1-2 ug/ml
Application Notes
ELISA: For coating, order this antibody without BSA
Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Sirtuin 1/SIRT1
Long Name
Silent Mating Type Information Regulation 2 Homolog
Alternate Names
SIR2-like protein 1, SIR2alpha, SIR2L1, SIRT1
Gene Symbol
SIRT1
Additional Sirtuin 1/SIRT1 Products
Product Documents for Sirtuin 1/SIRT1 Antibody (PCRP-SIRT1-1E11) - Azide and BSA Free
Product Specific Notices for Sirtuin 1/SIRT1 Antibody (PCRP-SIRT1-1E11) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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