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Sirtuin 1/SIRT1 [p Ser47] Antibody (SR1063)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21546

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-21546-100ul
NBP3-21546-50ul

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1063

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for Sirtuin 1/SIRT1 [p Ser47] Antibody (SR1063)

Immunogen

A synthesized peptide derived from human phospho-Sirtuin 1/SIRT1 [Ser47] (Uniprot #: Q96EB6)

Modification

p Ser47

Specificity

Detects endogenous levels of phospho-Sirtuin 1/SIRT1 [Ser47]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Sirtuin 1/SIRT1 [p Ser47] Antibody (SR1063)

Western Blot: Sirtuin 1/SIRT1 [p Ser 47] Antibody (SR1063) [NBP3-21546] -

Western Blot: Sirtuin 1/SIRT1 [p Ser 47] Antibody (SR1063) [NBP3-21546] - Western blot analysis using NBP3-21546 in (1) HEK293 cell lysate; (2) HEK293 cell lysate treated with LP.

Immunohistochemistry: Sirtuin 1/SIRT1 [p Ser 47] Antibody (SR1063) [NBP3-21546] -

Immunohistochemistry: Sirtuin 1/SIRT1 [p Ser 47] Antibody (SR1063) [NBP3-21546] - Immunohistochemical analysis of paraffin-embedded human colon, using NBP3-21546.

Applications for Sirtuin 1/SIRT1 [p Ser47] Antibody (SR1063)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:1000-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Sirtuin 1/SIRT1

The S. cerevisiae silent information regulator 2 (Sir2) protein is an NAD+-dependent histone deacetylase (HDACs) that plays a critical role in transcriptional silencing, genome stability, and longevity (1-2). Part of the Sirtuin family (Sir2, Sir3, Sir3), yeast Sir2 is the founding member of Class III HDACs. A human homologue of Sir2 , SIRT1, regulates the activity of the p53 tumor suppressor and inhibits apoptosis (3-4).

Long Name

Silent Mating Type Information Regulation 2 Homolog

Alternate Names

SIR2-like protein 1, SIR2alpha, SIR2L1, SIRT1

Gene Symbol

SIRT1

Additional Sirtuin 1/SIRT1 Products

Product Documents for Sirtuin 1/SIRT1 [p Ser47] Antibody (SR1063)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Sirtuin 1/SIRT1 [p Ser47] Antibody (SR1063)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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