Sirtuin 1/SIRT1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87038

Novus Biologicals, part of Bio-Techne
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NBP1-87038
NBP1-87038-25ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Sirtuin 1/SIRT1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: IVTLLDQAAKSNDDLDVSESKGCMEEKPQEVQTSRNVESIAEQMENPDLKNVGSSTGEKNERTSVAGTVRKCWPNRVAKEQISRRLDGNQYLFLPPNRYIFHGAEVYSDSEDDVLSSSSCGSNSD

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (80%). Human reactivity reported in scientific literature (PMID: 25146318). Mouse reactivity reported in the scientific literature (PMID: 26046931).

Marker

Nuclear Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Sirtuin 1/SIRT1 Antibody

Immunocytochemistry/ Immunofluorescence: Sirtuin 1/SIRT1 Antibody [NBP1-87038]

Immunocytochemistry/ Immunofluorescence: Sirtuin 1/SIRT1 Antibody [NBP1-87038]

Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody [NBP1-87038] - Staining of human cell line U-251 MG shows localization to nucleus & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Sirtuin 1/SIRT1 Antibody [NBP1-87038]

Immunohistochemistry-Paraffin: Sirtuin 1/SIRT1 Antibody [NBP1-87038]

Immunohistochemistry-Paraffin: Sirtuin 1/SIRT1 Antibody [NBP1-87038] - Staining of human lymph node shows moderate to strong nuclear positivity in germinal center cells.
Immunohistochemistry-Paraffin: Sirtuin 1/SIRT1 Antibody [NBP1-87038]

Immunohistochemistry-Paraffin: Sirtuin 1/SIRT1 Antibody [NBP1-87038]

Immunohistochemistry-Paraffin: Sirtuin 1/SIRT1 Antibody [NBP1-87038] - Saining of human liver shows no positivity in hepatocytes as expected.
View 2 more images

Applications for Sirtuin 1/SIRT1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. Use in Western blot reported in scientific literature (PMID 26791201).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Sirtuin 1/SIRT1

The S. cerevisiae silent information regulator 2 (Sir2) protein is an NAD+-dependent histone deacetylase (HDACs) that plays a critical role in transcriptional silencing, genome stability, and longevity (1-2). Part of the Sirtuin family (Sir2, Sir3, Sir3), yeast Sir2 is the founding member of Class III HDACs. A human homologue of Sir2 , SIRT1, regulates the activity of the p53 tumor suppressor and inhibits apoptosis (3-4).

Long Name

Silent Mating Type Information Regulation 2 Homolog

Alternate Names

SIR2-like protein 1, SIR2alpha, SIR2L1, SIRT1

Gene Symbol

SIRT1

Additional Sirtuin 1/SIRT1 Products

Product Documents for Sirtuin 1/SIRT1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Sirtuin 1/SIRT1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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