Sirtuin 3/SIRT3 Antibody (7O4G7)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16083
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockout Validated, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 7O4G7
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 300-399 of human Sirtuin 3/SIRT3 (NP_036371.1). QRFLLHVVDFPMADLLLILGTSLEVEPFASLTEAVRSSVPRLLINRDLVGPLAWHPRSRDVAQLGDVVHGVESLVELLGWTEEMRDLVQRETGKLDGPDK
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Sirtuin 3/SIRT3 Antibody (7O4G7)
![Immunohistochemistry-Paraffin: Sirtuin 3/SIRT3 Antibody (7O4G7) [NBP3-16083]](https://resources.bio-techne.com/images/products/Sirtuin-3-SIRT3-Antibody-7O4G7-Immunohistochemistry-Paraffin-NBP3-16083-img0002.jpg "Immunohistochemistry-Paraffin: Sirtuin 3/SIRT3 Antibody (7O4G7) [NBP3-16083]")
Immunohistochemistry-Paraffin: Sirtuin 3/SIRT3 Antibody (7O4G7) [NBP3-16083]
Immunohistochemistry-Paraffin: Sirtuin 3/SIRT3 Antibody (7O4G7) [NBP3-16083] - Immunohistochemistry of paraffin-embedded mouse kidney using [KO Validated] Sirtuin 3/SIRT3 Rabbit mAb (NBP3-16083) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Western Blot: Sirtuin 3/SIRT3 Antibody (7O4G7) [NBP3-16083] -
Western Blot: Sirtuin 3/SIRT3 Antibody (7O4G7) [NBP3-16083] - Western blot analysis of lysates from wild type (WT) and Sirtuin 3/SIRT3 knockout (KO) 293T cells using [KO Validated] Sirtuin 3/SIRT3 Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western Blot: Sirtuin 3/SIRT3 Antibody (7O4G7) [NBP3-16083] -
Western Blot: Sirtuin 3/SIRT3 Antibody (7O4G7) [NBP3-16083] - Western blot analysis of various lysates using [KO Validated] Sirtuin 3/SIRT3 Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Applications for Sirtuin 3/SIRT3 Antibody (7O4G7)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Immunoprecipitation
1:500 - 1:1000
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Sirtuin 3/SIRT3
Long Name
Silent Mating Type Information Regulation 3 Homolog
Alternate Names
SIR2-like protein 3, SIR2L3, SIRT3
Gene Symbol
SIRT3
Additional Sirtuin 3/SIRT3 Products
Product Documents for Sirtuin 3/SIRT3 Antibody (7O4G7)
Product Specific Notices for Sirtuin 3/SIRT3 Antibody (7O4G7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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