Sirtuin 3/SIRT3 Antibody (7O4G7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16083

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16083-100ul
NBP3-16083-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7O4G7

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Sirtuin 3/SIRT3 Antibodies »

Product Summary for Sirtuin 3/SIRT3 Antibody (7O4G7)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 300-399 of human Sirtuin 3/SIRT3 (NP_036371.1). QRFLLHVVDFPMADLLLILGTSLEVEPFASLTEAVRSSVPRLLINRDLVGPLAWHPRSRDVAQLGDVVHGVESLVELLGWTEEMRDLVQRETGKLDGPDK

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Sirtuin 3/SIRT3 Antibody (7O4G7)

Immunohistochemistry-Paraffin: Sirtuin 3/SIRT3 Antibody (7O4G7) [NBP3-16083]

Immunohistochemistry-Paraffin: Sirtuin 3/SIRT3 Antibody (7O4G7) [NBP3-16083]

Immunohistochemistry-Paraffin: Sirtuin 3/SIRT3 Antibody (7O4G7) [NBP3-16083] - Immunohistochemistry of paraffin-embedded mouse kidney using [KO Validated] Sirtuin 3/SIRT3 Rabbit mAb (NBP3-16083) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunoprecipitation: Sirtuin 3/SIRT3 Antibody (7O4G7) [NBP3-16083]

Immunoprecipitation: Sirtuin 3/SIRT3 Antibody (7O4G7) [NBP3-16083]

Immunoprecipitation: Sirtuin 3/SIRT3 Antibody (7O4G7) [NBP3-16083] - Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug [KO Validated] Sirtuin 3/SIRT3 antibody (NBP3-16083). Western blot was performed from the immunoprecipitate using [KO Validated] Sirtuin 3/SIRT3 antibody (NBP3-16083) at a dilition of 1:1000.
Western Blot- Sirtuin 3/SIRT3 Antibody (7O4G7) [NBP3-16083] -

Western Blot- Sirtuin 3/SIRT3 Antibody (7O4G7) [NBP3-16083] -

Western Blot- Sirtuin 3/SIRT3 Antibody (7O4G7) [NBP3-16083] - Analysis of various lysates, using SIRT3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Applications for Sirtuin 3/SIRT3 Antibody (7O4G7)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Sirtuin 3/SIRT3

SIRT3 is expected to recognize both reported isoforms (NP_036371.1 and NP_001017524.1).

Long Name

Silent Mating Type Information Regulation 3 Homolog

Alternate Names

SIR2-like protein 3, SIR2L3, SIRT3

Gene Symbol

SIRT3

Additional Sirtuin 3/SIRT3 Products

Product Documents for Sirtuin 3/SIRT3 Antibody (7O4G7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Sirtuin 3/SIRT3 Antibody (7O4G7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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