Sirtuin 3/SIRT3 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-02984

Novus Biologicals, part of Bio-Techne
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NBP3-02984-100ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Sirtuin 3/SIRT3 Antibodies »

Product Summary for Sirtuin 3/SIRT3 Antibody - Azide and BSA Free

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 299-399 of human Sirtuin 3/SIRT3 (NP_036371.1). PQRFLLHVVDFPMADLLLILGTSLEVEPFASLTEAVRSSVPRLLINRDLVGPLAWHPRSRDVAQLGDVVHGVESLVELLGWTEEMRDLVQRETGKLDGPDK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Sirtuin 3/SIRT3 Antibody - Azide and BSA Free

Western Blot: Sirtuin 3/SIRT3 AntibodyAzide and BSA Free [NBP3-02984]

Western Blot: Sirtuin 3/SIRT3 AntibodyAzide and BSA Free [NBP3-02984]

Western Blot: Sirtuin 3/SIRT3 Antibody [NBP3-02984] - Analysis of extracts of various cell lines, using SIRT3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.
Immunocytochemistry/ Immunofluorescence: Sirtuin 3/SIRT3 Antibody - Azide and BSA Free [NBP3-02984]

Immunocytochemistry/ Immunofluorescence: Sirtuin 3/SIRT3 Antibody - Azide and BSA Free [NBP3-02984]

Immunocytochemistry/Immunofluorescence: Sirtuin 3/SIRT3 Antibody [NBP3-02984] - Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] Sirtuin 3/SIRT3 Rabbit pAb (NBP3-02984) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: Sirtuin 3/SIRT3 Antibody - Azide and BSA Free [NBP3-02984]

Immunohistochemistry-Paraffin: Sirtuin 3/SIRT3 Antibody - Azide and BSA Free [NBP3-02984]

Immunohistochemistry-Paraffin: Sirtuin 3/SIRT3 Antibody [NBP3-02984] - Mouse kidney using SIRT3 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for Sirtuin 3/SIRT3 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:20 - 1:50

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Sirtuin 3/SIRT3

SIRT3 is expected to recognize both reported isoforms (NP_036371.1 and NP_001017524.1).

Long Name

Silent Mating Type Information Regulation 3 Homolog

Alternate Names

SIR2-like protein 3, SIR2L3, SIRT3

Gene Symbol

SIRT3

Additional Sirtuin 3/SIRT3 Products

Product Documents for Sirtuin 3/SIRT3 Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Sirtuin 3/SIRT3 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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