Sirtuin 5/SIRT5 Antibody (OTI2F1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-01003
![Flow Cytometry: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003]](https://resources.bio-techne.com/images/products/Sirtuin-5-SIRT5-Antibody-2F1-Flow-Cytometry-NBP2-01003-img0012.jpg "Flow Cytometry: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003]")
![Western Blot: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003]](https://resources.bio-techne.com/images/products/Sirtuin-5-SIRT5-Antibody-OTI2F1-Western-Blot-NBP2-01003-img0017.jpg "Western Blot: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI2F1
Concentration
0.24 mg/ml
Product Summary for Sirtuin 5/SIRT5 Antibody (OTI2F1)
Immunogen
Full length human recombinant protein of human SIRT5(NP_036373) produced in HEK293 cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
33.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Sirtuin 5/SIRT5 Antibody (OTI2F1)
Western Blot: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003]
Western Blot: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003] - Equivalent amounts of cell lysates (10ug per lane) of wild-type HeLa cells and SIRT5-Knockout HeLa cells were separated by SDS-PAGE and immunoblotted with anti-SIRT5 monoclonal antibody (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.![Immunocytochemistry/ Immunofluorescence: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003]](https://resources.bio-techne.com/images/products/Sirtuin-5-SIRT5-Antibody-2F1-Immunocytochemistry-Immunofluorescence-NBP2-01003-img0013.jpg "Immunocytochemistry/ Immunofluorescence: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003]")
Immunocytochemistry/ Immunofluorescence: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003]
Immunocytochemistry/Immunofluorescence: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003] - Sirtuin 5/SIRT5 Antibody (2F1) [NBP2-01003] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SIRT5.![Immunohistochemistry-Paraffin: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003]](https://resources.bio-techne.com/images/products/Sirtuin-5-SIRT5-Antibody-2F1-Immunohistochemistry-Paraffin-NBP2-01003-img0014.jpg "Immunohistochemistry-Paraffin: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003]")
Immunohistochemistry-Paraffin: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003]
Immunohistochemistry-Paraffin: Sirtuin 5/SIRT5 Antibody (2F1) [NBP2-01003] - Staining of paraffin-embedded Human Kidney tissue using anti-SIRT5 mouse monoclonal antibody.![Western Blot: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003]](https://resources.bio-techne.com/images/products/Sirtuin-5-SIRT5-Antibody-2F1-Western-Blot-NBP2-01003-img0015.jpg "Western Blot: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003]")
![Western Blot: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003]](https://resources.bio-techne.com/images/products/Sirtuin-5-SIRT5-Antibody-2F1-Western-Blot-NBP2-01003-img0011.jpg "Western Blot: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003]")
Applications for Sirtuin 5/SIRT5 Antibody (OTI2F1)
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:150
Immunohistochemistry-Paraffin
1:150
Western Blot
1:500-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.24 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Sirtuin 5/SIRT5
Long Name
Silent Mating Type Information Regulation 5 Homolog
Alternate Names
SIR2L5, SIRT5
Entrez Gene IDs
23408 (Human)
Gene Symbol
SIRT5
Additional Sirtuin 5/SIRT5 Products
Product Documents for Sirtuin 5/SIRT5 Antibody (OTI2F1)
Product Specific Notices for Sirtuin 5/SIRT5 Antibody (OTI2F1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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