Sirtuin 5/SIRT5 Antibody (OTI2F1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-01003

Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2F1

Concentration

0.24 mg/ml

View all Sirtuin 5/SIRT5 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human SIRT5(NP_036373) produced in HEK293 cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

33.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Sirtuin 5/SIRT5 Antibody (OTI2F1)

Western Blot: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003]

Western Blot: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003]

Western Blot: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003] - Equivalent amounts of cell lysates (10ug per lane) of wild-type HeLa cells and SIRT5-Knockout HeLa cells were separated by SDS-PAGE and immunoblotted with anti-SIRT5 monoclonal antibody (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.
Immunocytochemistry/ Immunofluorescence: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003]

Immunocytochemistry/ Immunofluorescence: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003]

Immunocytochemistry/Immunofluorescence: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003] - Sirtuin 5/SIRT5 Antibody (2F1) [NBP2-01003] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SIRT5.
Immunohistochemistry-Paraffin: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003]

Immunohistochemistry-Paraffin: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003]

Immunohistochemistry-Paraffin: Sirtuin 5/SIRT5 Antibody (2F1) [NBP2-01003] - Staining of paraffin-embedded Human Kidney tissue using anti-SIRT5 mouse monoclonal antibody.
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Applications for Sirtuin 5/SIRT5 Antibody (OTI2F1)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.24 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Sirtuin 5/SIRT5

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class III of the sirtuin family. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq].

Long Name

Silent Mating Type Information Regulation 5 Homolog

Alternate Names

SIR2L5, SIRT5

Entrez Gene IDs

23408 (Human)

Gene Symbol

SIRT5

Additional Sirtuin 5/SIRT5 Products

Product Documents for Sirtuin 5/SIRT5 Antibody (OTI2F1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Sirtuin 5/SIRT5 Antibody (OTI2F1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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