Sirtuin 5/SIRT5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89535

Novus Biologicals, part of Bio-Techne
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NBP1-89535
NBP1-89535-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (91%), Rat (90%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Sirtuin 5/SIRT5 Antibodies »

Product Summary for Sirtuin 5/SIRT5 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: IDELHRKAGTKNLLEIHGSLFKTRCTSCGVVAENYKSPICPALSGKGAPEPGTQDASIPVEKLPRCEEAGCGGLLRPHVVW

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Sirtuin 5/SIRT5 Antibody

Western Blot: Sirtuin 5/SIRT5 Antibody [NBP1-89535]

Western Blot: Sirtuin 5/SIRT5 Antibody [NBP1-89535]

Western Blot: Sirtuin 5/SIRT5 Antibody [NBP1-89535] - Analysis in human cell line EFO-21.
Immunocytochemistry/ Immunofluorescence: Sirtuin 5/SIRT5 Antibody [NBP1-89535]

Immunocytochemistry/ Immunofluorescence: Sirtuin 5/SIRT5 Antibody [NBP1-89535]

Immunocytochemistry/Immunofluorescence: Sirtuin 5/SIRT5 Antibody [NBP1-89535] - Staining of human cell line A-431 shows localization to mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Sirtuin 5/SIRT5 Antibody [NBP1-89535]

Immunohistochemistry-Paraffin: Sirtuin 5/SIRT5 Antibody [NBP1-89535]

Immunohistochemistry-Paraffin: Sirtuin 5/SIRT5 Antibody [NBP1-89535] - Staining of human testis shows strong granular positivity in cytoplasm in cells in seminiferous ducts.
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Applications for Sirtuin 5/SIRT5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Sirtuin 5/SIRT5

SIRT5 encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class III of the sirtuin family. Alternative splicing of this gene results in two transcript variants.

Long Name

Silent Mating Type Information Regulation 5 Homolog

Alternate Names

SIR2L5, SIRT5

Gene Symbol

SIRT5

Additional Sirtuin 5/SIRT5 Products

Product Documents for Sirtuin 5/SIRT5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Sirtuin 5/SIRT5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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