SIX1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84264
Key Product Details
Validated by
Knockout/Knockdown, Orthogonal Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Predicted:
Rat (99%). Backed by our 100% Guarantee.
Applications
Validated:
Chromatin Immunoprecipitation, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot
Cited:
Chemotaxis, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: CFKEKSRGVLREWYAHNPYPSPREKRELAEATGLTTTQVSNWFKNRRQRDRAAEAKERENTENNNSSSNKQNQLSPLEGGKPLMSSSEEEFSPPQSPDQNSVLLLQGNMGHARSSNYSLPGLTASQPSHGLQTHQHQLQDS
Reactivity Notes
Use in Mouse reported in scientific literature (PMID:24852826).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SIX1 Antibody
Immunocytochemistry/ Immunofluorescence: SIX1 Antibody [NBP1-84264]
Immunocytochemistry/Immunofluorescence: SIX1 Antibody [NBP1-84264] - Staining of human cell line A-431 shows localization to nucleus & nucleoli. Antibody staining is shown in green.Applications for SIX1 Antibody
Application
Recommended Usage
Chromatin Immunoprecipitation
Reported in scientific literature (PMID: 24852826)
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:500-1:1000
Immunohistochemistry-Paraffin
1:500-1:1000
Western Blot
0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SIX1
Alternate Names
BOS3, deafness, autosomal dominant 23, DFNA23, homeobox protein SIX1, sine oculis homeobox (Drosophila) homolog 1, Sine oculis homeobox homolog 1, sine oculis homeobox homolog 1 (Drosophila), SIX homeobox 1, TIP39
Entrez Gene IDs
6495 (Human)
Gene Symbol
SIX1
UniProt
Additional SIX1 Products
Product Documents for SIX1 Antibody
Product Specific Notices for SIX1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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