SIX1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84264

Novus Biologicals, part of Bio-Techne
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NBP1-84264
NBP1-84264-25ul
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Rat (99%). Backed by our 100% Guarantee.

Applications

Validated:

Chromatin Immunoprecipitation, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Cited:

Chemotaxis, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SIX1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: CFKEKSRGVLREWYAHNPYPSPREKRELAEATGLTTTQVSNWFKNRRQRDRAAEAKERENTENNNSSSNKQNQLSPLEGGKPLMSSSEEEFSPPQSPDQNSVLLLQGNMGHARSSNYSLPGLTASQPSHGLQTHQHQLQDS

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:24852826).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SIX1 Antibody

Immunohistochemistry-Paraffin: SIX1 Antibody [NBP1-84264]

Immunohistochemistry-Paraffin: SIX1 Antibody [NBP1-84264]

Immunohistochemistry-Paraffin: SIX1 Antibody [NBP1-84264] - Staining in human skeletal muscle and liver tissues . Corresponding SIX1 RNA-seq data are presented for the same tissues.
Western Blot: SIX1 Antibody [NBP1-84264]

Western Blot: SIX1 Antibody [NBP1-84264]

Western Blot: SIX1 Antibody [NBP1-84264] - Analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Immunocytochemistry/ Immunofluorescence: SIX1 Antibody [NBP1-84264]

Immunocytochemistry/ Immunofluorescence: SIX1 Antibody [NBP1-84264]

Immunocytochemistry/Immunofluorescence: SIX1 Antibody [NBP1-84264] - Staining of human cell line A-431 shows localization to nucleus & nucleoli. Antibody staining is shown in green.
View 4 more images

Applications for SIX1 Antibody

Application
Recommended Usage

Chromatin Immunoprecipitation

Reported in scientific literature (PMID: 24852826)

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500-1:1000

Immunohistochemistry-Paraffin

1:500-1:1000

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SIX1

The protein encoded by the SIX1 gene is a homeobox protein that is similar to the Drosophila 'sine oculis' gene product. This gene is found in a cluster of related genes on chromosome 14 and is thought to be involved in limb development. Defects in this gene are a cause of autosomal dominant deafness type 23 (DFNA23) and branchiootic syndrome type 3 (BOS3). (provided by RefSeq)

Alternate Names

BOS3, deafness, autosomal dominant 23, DFNA23, homeobox protein SIX1, sine oculis homeobox (Drosophila) homolog 1, Sine oculis homeobox homolog 1, sine oculis homeobox homolog 1 (Drosophila), SIX homeobox 1, TIP39

Entrez Gene IDs

6495 (Human)

Gene Symbol

SIX1

UniProt

Q15475 (Human)

Additional SIX1 Products

Product Documents for SIX1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SIX1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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