Skp1 Antibody (10G5X5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16734

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16734-100ul
NBP3-16734-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 10G5X5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SKP1 Antibodies »

Product Summary for Skp1 Antibody (10G5X5)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 64-163 of human SKP1 (P63208). HHKDDPPPPEDDENKEKRTDDIPVWDQEFLKVDQGTLFELILAANYLDIKGLLDVTCKTVANMIKGKTPEEIRKTFNIKNDFTEEEEAQVRKENQWCEEK

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Skp1 Antibody (10G5X5)

Western Blot: Skp1 Antibody (10G5X5) [NBP3-16734]

Western Blot: Skp1 Antibody (10G5X5) [NBP3-16734]

Western Blot: Skp1 Antibody (10G5X5) [NBP3-16734] - Western blot analysis of extracts of various cell lines, using Skp1 Rabbit mAb (NBP3-16734) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunocytochemistry/ Immunofluorescence: Skp1 Antibody (10G5X5) [NBP3-16734]

Immunocytochemistry/ Immunofluorescence: Skp1 Antibody (10G5X5) [NBP3-16734]

Immunocytochemistry/Immunofluorescence: Skp1 Antibody (10G5X5) [NBP3-16734] - Immunofluorescence analysis of NIH-3T3 cells using Skp1 Rabbit mAb (NBP3-16734) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for Skp1 Antibody (10G5X5)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SKP1

Skp1 encodes a component of SCF complexes, which are composed of this protein, cullin 1, a ring-box protein, and one member of the F-box family of proteins. This protein binds directly to the F-box motif found in F-box proteins. SCF complexes are involved in the regulated ubiquitination of specific protein substrates, which targets them for degradation by the proteosome. Specific F-box proteins recognize different target protein(s), and many specific SCF substrates have been identified including regulators of cell cycle progression and development. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7.

Long Name

S-phase Kinase Associated Protein 1

Alternate Names

OCP2, p19a, p19Skp1, SKP1A

Gene Symbol

SKP1

Additional SKP1 Products

Product Documents for Skp1 Antibody (10G5X5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Skp1 Antibody (10G5X5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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