SLBP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12936

Novus Biologicals, part of Bio-Techne
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NBP3-12936
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Predicted:

Bovine (93%), Chimpanzee (100%), Mouse (95%), Porcine (90%), Rat (93%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SLBP Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human SLBP. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for SLBP Antibody

Western Blot: SLBP Antibody [NBP3-12936]

Western Blot: SLBP Antibody [NBP3-12936]

Western Blot: SLBP Antibody [NBP3-12936] - HepG2 whole cell and cytoplasm extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SLBP antibody (NBP3-12936) diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: SLBP Antibody [NBP3-12936]

Immunocytochemistry/ Immunofluorescence: SLBP Antibody [NBP3-12936]

Immunocytochemistry/Immunofluorescence: SLBP Antibody [NBP3-12936] - SLBP antibody detects SLBP protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: Hep G2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SLBP protein stained by SLBP antibody (NBP3-12936) diluted at 1:500. Blue: Hoechst 33342 staining.
Knockdown Validated: SLBP Antibody [NBP3-12936]

Knockdown Validated: SLBP Antibody [NBP3-12936]

Knockdown Validated: SLBP Antibody [NBP3-12936] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SLBP antibody (NBP3-12936) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Applications for SLBP Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SLBP

SLBP encodes a protein that binds to the stem-loop structure in replication-dependent histone mRNAs. Histone mRNAs do not contain introns or polyadenylation signals, and are processed by endonucleolytic cleavage. The stem-loop structure is essential for efficient processing but this structure also controls the transport, translation and stability of histone mRNAs. Expression of the protein is regulated during the cell cycle, increasing more than 10-fold during the latter part of G1.

Alternate Names

histone, histone binding protein, histone RNA hairpin-binding protein, histone stem-loop binding protein, Histone stem-loop-binding protein, stem-loop binding protein

Gene Symbol

SLBP

Additional SLBP Products

Product Documents for SLBP Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SLBP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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