Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine

Cited:

Mouse

Applications

Validated:

Electron Microscopy, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all SLC12A3 Antibodies »

Product Specifications

Immunogen

Produced against a synthetic peptide mapping to a segment of rat NCC (amino acids 74-95), N-terminal

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31630621).

Localization

Membrane

Specificity

Detects approx 160kDa.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SLC12A3 Antibody - BSA Free

Western Blot: SLC12A3 Antibody [NBP1-44270]

Western Blot: SLC12A3 Antibody [NBP1-44270]

Western Blot: SLC12A3 Antibody [NBP1-44270] - Western blot analysis of Rat tissue lysates showing detection of SLC12A3 protein using Rabbit Anti-SLC12A3 Polyclonal Antibody (NBP1-44270). Primary Antibody: Rabbit Anti-SLC12A3 Polyclonal Antibody (NBP1-44270) at 1:1000.
Immunohistochemistry: SLC12A3 Antibody [NBP1-44270]

Immunohistochemistry: SLC12A3 Antibody [NBP1-44270]

Immunohistochemistry: SLC12A3 Antibody [NBP1-44270] - Immunohistochemistry analysis using Rabbit Anti-SLC12A3 Polyclonal Antibody (NBP1-44270). Tissue: kidney tissue. Species: Rat. Primary Antibody: Rabbit Anti-SLC12A3 Polyclonal Antibody (NBP1-44270) at 1:200. Secondary Antibody: FITC Goat Anti-Rabbit (green).

Applications for SLC12A3 Antibody - BSA Free

Application
Recommended Usage

Electron Microscopy

1:10-1:500

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Western Blot

1:1000
Application Notes
1 ug/ml of NCC Antibody was sufficient for detection of NCC3 in 10 ug of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary Antibody.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SLC12A3

NCC, a thiazide-sensitive NaCl co-transporter, is found on the apical membrane of the distal convoluted tubule, where it is the principal mediator of Na+ and Clreabsorption in this segment of the nephron. It is activated by phosphorylation, and has been implicated in renal NaCl and K+ homeostasis (1). Regulation of NCC expression and phosphorylation by dietary NACl restriction appears to involve SGK1(1). In experiments with angiotensin II-infused mice, increased sensitivity to Ang II may involved over-activity of NCC (2). Therefore, NCC is the target of thiazide diuretics used in the treatment of hypertension (1). Molecular experiments have also shown that NCC has been detected in the lens cortex, core and fiber cells of a rat (3).

Long Name

Solute Carrier Family 12 (Sodium/Chloride Transporters) Member 3

Alternate Names

NCC, NCCT, TSC

Entrez Gene IDs

6559 (Human)

Gene Symbol

SLC12A3

UniProt

NP_062218 (Rat)

Additional SLC12A3 Products

Product Documents for SLC12A3 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SLC12A3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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