SLC7A5/LAT1 Antibody (EP3-1) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20116
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Flow Cytometry, Functional, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # EP3-1
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for SLC7A5/LAT1 Antibody (EP3-1) - Chimeric - Azide and BSA Free
Immunogen
Immunised mice and constructed IgG1 Fab phage display libraries from the spleen cells. SLC7A5/LAT1-reactive recombinant Fab fragments were retrieved by epitope peptide panning.
Epitope
Shown to bind to epitopes of sequences; LLHGDFHAFSAG-GGGC-NH2, LLHGDFH-GGGC-HN2, and FHAFSAG-GGGC-NH2.
Specificity
Shows no reactivity to live HeLa cells by ICC/IF.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images for SLC7A5/LAT1 Antibody (EP3-1) - Chimeric - Azide and BSA Free
Immunocytochemistry/Immunofluorescence: SLC7A5/LAT1 Antibody (EP3-1) - Chimeric - Azide and BSA Free [NBP3-20116] -
Immunocytochemistry/Immunofluorescence: SLC7A5/LAT1 Antibody (EP3-1) - Chimeric - Azide and BSA Free [NBP3-20116] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells stained with the chimeric rabbit IgG version of EP3-1 (NBP3-20116) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20116, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.
Flow Cytometry: SLC7A5/LAT1 Antibody (EP3-1) - Chimeric - Azide and BSA Free [NBP3-20116] -
Flow Cytometry: SLC7A5/LAT1 Antibody (EP3-1) - Chimeric - Azide and BSA Free [NBP3-20116] - Paraformaldehyde fixed MCF7 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of EP3-1 (NBP3-20116, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.Applications for SLC7A5/LAT1 Antibody (EP3-1) - Chimeric - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Functional
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: SLC7A5/LAT1
Long Name
Solute Carrier Family 7 Member 5
Alternate Names
4F2LC, CD98lc, E16, LAT1, MPE16, TA1
Gene Symbol
SLC7A5
Additional SLC7A5/LAT1 Products
Product Documents for SLC7A5/LAT1 Antibody (EP3-1) - Chimeric - Azide and BSA Free
Product Specific Notices for SLC7A5/LAT1 Antibody (EP3-1) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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