SMAC/Diablo Antibody (S08-6A3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-19775

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-19775-100ul
NBP3-19775-50ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S08-6A3

Concentration

0.3 mg/ml

View all SMAC/Diablo Antibodies »

Product Specifications

Immunogen

A synthetic peptide of human SMAC/Diablo (Uniprot # Q9NR28)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SMAC/Diablo Antibody (S08-6A3)

Western Blot: SMAC/Diablo Antibody (S08-6A3) [NBP3-19775]

Western Blot: SMAC/Diablo Antibody (S08-6A3) [NBP3-19775]

Western Blot: SMAC/Diablo Antibody (S08-6A3) [NBP3-19775] - Western blot detection of SMAC/Diablo in K562, Hela cell lysates using NBP3-19775 (1:1000 diluted). Predicted band size: 27kDa. Observed band size: 21kDa.
Immunocytochemistry/ Immunofluorescence: SMAC/Diablo Antibody (S08-6A3) [NBP3-19775]

Immunocytochemistry/ Immunofluorescence: SMAC/Diablo Antibody (S08-6A3) [NBP3-19775]

Immunocytochemistry/Immunofluorescence: SMAC/Diablo Antibody (S08-6A3) [NBP3-19775] - Immunocytochemistry of SMAC/Diablo (green) in Hela cells using NBP3-19775 at dilution 1/200, and DAPI (blue)
Immunohistochemistry-Paraffin: SMAC/Diablo Antibody (S08-6A3) [NBP3-19775]

Immunohistochemistry-Paraffin: SMAC/Diablo Antibody (S08-6A3) [NBP3-19775]

Immunohistochemistry-Paraffin: SMAC/Diablo Antibody (S08-6A3) [NBP3-19775] - Immunohistochemistry of SMAC/Diablo in paraffin-embedded Human tonsil using NBP3-19775 at dilution 1/5

Applications for SMAC/Diablo Antibody (S08-6A3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1/100

Immunohistochemistry

1/20-1/100

Western Blot

1/1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SMAC/Diablo

Smac/DIABLO is a mitochondrial protein which activates various forms of apoptosis. Specifically, Smac exits the mitochondria and enters the cytosol where it initiates apoptosis by neutralizing members of the IAP family of apoptosis inhibitory proteins. Mitochondrial-mediated apoptosis is important in animal development and tissue homeostasis, with alterations resulting in a range of malignant disorders. Upon apoptotic stimuli, the mitochondrial proteins cytochrome c and Smac/DIABLO are released into the cytosol. The release of these proteins, however, occurs via different mechanisms.

Long Name

Second Mitochondria-derived Activator of Caspase

Alternate Names

Diablo

Gene Symbol

DIABLO

Additional SMAC/Diablo Products

Product Documents for SMAC/Diablo Antibody (S08-6A3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SMAC/Diablo Antibody (S08-6A3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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