SMAD1/5/9 Antibody (RM487)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18834

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-18834
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM487

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SMAD1/5/9 Antibodies »

Product Summary for SMAD1/5/9 Antibody (RM487)

Immunogen

A phospho-peptide corresponding to human Phospho-SMAD1 [Ser463/465]/ SMAD5 [Ser463/465]/ SMAD9 [Ser465/467]

Specificity

This antibody reacts to SMAD1 and SMAD5 when phosphorylated at Ser463/465 and SMAD9 when phosphorylated at Ser465/467. There is no cross-reactivity to SMAD1, SMAD5, or SMAD9 that are not phosphorylated.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SMAD1/5/9 Antibody (RM487)

SMAD1/5/9 Antibody (RM487)

Western Blot: SMAD1/5/9 Antibody (RM487)[NBP3-18834]

Western Blot: SMAD1/5/9 Antibody (RM487)[NBP3-18834]Western Blot of mouse liver tissue lysate, using Anti-Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad9 (Ser465/467) rabbit monoclonal antibody (clone RM487) at a 1:1000 dilution. The phospho-specificity of RM487 was verified by peptide blocking using a control nonphospho-peptide or phosphor-peptide targeting residue Ser463/465 of Smad1 and Smad5 and residue Ser465/467 of Smad9.

Applications for SMAD1/5/9 Antibody (RM487)

Application
Recommended Usage

Western Blot

1:1000-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50% Glycerol/PBS, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: SMAD1/5/9

Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFbeta/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MadH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFbeta and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFbeta signaling by interfering with TGFbeta-mediated phosphorylation of other Smad family members.

Alternate Names

BSP1, BSP-1, hSMAD1, JV4-1SMAD, mothers against DPP homolog 1 (Drosophila), MAD homolog 1, MADH1JV41, MADR1MAD, mothers against decapentaplegic homolog 1 (Drosophila), Mad-related protein 1, mothers against decapentaplegic homolog 1, Mothers against DPP homolog 1, SMAD family member 1SMAD 1, SMAD, mothers against DPP homolog 1, Smad1, TGF-beta signaling protein 1, transforming growth factor-beta signaling protein 1

Gene Symbol

SMAD1

Additional SMAD1/5/9 Products

Product Documents for SMAD1/5/9 Antibody (RM487)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SMAD1/5/9 Antibody (RM487)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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