Smad1 Antibody (2E9)

Novus Biologicals, part of Bio-Techne | Catalog # H00004086-M03

Novus Biologicals, part of Bio-Techne
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H00004086-M03
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2E9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Smad1 Antibodies »

Product Specifications

Immunogen

SMAD1 (AAH01878.1, 1 a.a. ~ 465 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MNVTSLFSFTSPAVKRLLGWKQGDEEEKWAEKAVDALVKKLKKKKGAMEELEKALSCPGQPSNCVTIPRSLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKPLECCEFPFGSKQKEVCINPYHYKRVESPVLPPVLVPRHSEYNPQHSLLAQFRNLGQNEPHMPLNATFPDSFQQPNSHPFPHSPNSSYPNSPGSSSSTYPHSPTSSDPGSPFQMPADTPPPAYLPPEDPMTQDGSQPMDTNMMAPPLPSEINRGDVQAVAYEEPKHWCSIVYYELNNRVGEAFHASSTSVLVDGFTDPSNNKNRFCLGLLSNVNRNSTIENTRRHIGKGVHLYYVGGEVYAECLSDSSIFVQSRNCNYHHGFHPTTVCKIPSGCSLKIFNNQEFAQLLAQSVNHGFETVYELTKMCTIRMSFVKGWGAEYHRQDVTSTPCWIEIHLHGPLQWLDKVLTQMGSPHNPISSVS

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

SMAD1 (2E9)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Smad1 Antibody (2E9)

Western Blot: Smad1 Antibody (2E9) [H00004086-M03]

Western Blot: Smad1 Antibody (2E9) [H00004086-M03]

Western Blot: Smad1 Antibody (2E9) [H00004086-M03] - Analysis of SMAD1 expression in NIH/3T3 (Cat # L018V1).
Immunocytochemistry/ Immunofluorescence: Smad1 Antibody (2E9) [H00004086-M03]

Immunocytochemistry/ Immunofluorescence: Smad1 Antibody (2E9) [H00004086-M03]

Immunocytochemistry/Immunofluorescence: Smad1 Antibody (2E9) [H00004086-M03] - Analysis of monoclonal antibody to SMAD1 on HeLa cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: Smad1 Antibody (2E9) [H00004086-M03]

Immunohistochemistry-Paraffin: Smad1 Antibody (2E9) [H00004086-M03]

Immunohistochemistry-Paraffin: Smad1 Antibody (2E9) [H00004086-M03] - Analysis of monoclonal antibody to SMAD1 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml
View 5 more images

Applications for Smad1 Antibody (2E9)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Smad1

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.

Long Name

Mothers Against DPP Homolog 1

Alternate Names

BSP1, BSP-1, hSMAD1, JV4-1SMAD, mothers against DPP homolog 1 (Drosophila), MAD homolog 1, MADH1JV41, MADR1MAD, mothers against decapentaplegic homolog 1 (Drosophila), Mad-related protein 1, mothers against decapentaplegic homolog 1, Mothers against DPP homolog 1, SMAD family member 1SMAD 1, SMAD, mothers against DPP homolog 1, Smad1, TGF-beta signaling protein 1, transforming growth factor-beta signaling protein 1

Entrez Gene IDs

4086 (Human); 17125 (Mouse); 25671 (Rat)

Gene Symbol

SMAD1

OMIM

601595 (Human)

UniProt

AAH01878 (Human)

Additional Smad1 Products

Product Documents for Smad1 Antibody (2E9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Smad1 Antibody (2E9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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