Smad2/3 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15589

Novus Biologicals, part of Bio-Techne
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NBP3-15589-100ul
NBP3-15589-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Smad2/3 Antibodies »

Product Summary for Smad2/3 Antibody - Azide and BSA Free

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 150-270 of human Smad2/3 (NP_005893.1). EYAFNLKKDEVCVNPYHYQRVETPVLPPVLVPRHTEILTELPPLDDYTHSIPENTNFPAGIEPQSNYIPETPPPGYISEDGETSDQQLNQSMDTGSPAELSPTTLSPVNHSLDLQPVTYSE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Smad2/3 Antibody - Azide and BSA Free

Western Blot: Smad2/3 AntibodyAzide and BSA Free [NBP3-15589]

Western Blot: Smad2/3 AntibodyAzide and BSA Free [NBP3-15589]

Western Blot: Smad2/3 Antibody [NBP3-15589] - Western blot analysis of extracts of various cell lines, using Smad2/3 antibody (NBP3-15589) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Applications for Smad2/3 Antibody - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500 -1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 50% glycerol, pH7.3

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Smad2/3

Smad proteins are essential mediators of signal transduction by the TGF-beta superfamily from the cell surface into the nucleus. Once in the nucleus, Smad proteins target a number of DNA binding proteins to stimulate transcription. Smad4, also known as the common-mediator Smad (co-Smad), interacts with the receptor-regulated Smad proteins (R-SMAD). The R-SMAD class includes Smad2 and Smad3.

Long Name

Mothers Against DPP Homolog 2/3

Alternate Names

hMAD-2, hSMAD2, JV18, JV18-1SMAD, mothers against DPP homolog 2 (Drosophila), MAD homolog 2, Mad protein homolog, Mad, mothers against decapentaplegic homolog 2, MADH2MGC34440, MADR2MGC22139, Mad-related protein 2, mother against DPP homolog 2, mothers against decapentaplegic homolog 2, Mothers against DPP homolog 2, Sma- and Mad-related protein 2, SMAD 2, SMAD family member 2MAD, mothers against decapentaplegic homolog 2 (Drosophila), SMAD, mothers against DPP homolog 2, Smad2

Gene Symbol

SMAD2

Additional Smad2/3 Products

Product Documents for Smad2/3 Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Smad2/3 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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