Smad3 [p Ser423] Antibody (SR1995)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21516
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # SR1995
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from human phospho-Smad3 [Ser423, Ser425] (Uniprot #: P84022)
Modification
p Ser 423
Specificity
Detects endogenous levels of total phospho-Smad3 [Ser423, Ser425]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Smad3 [p Ser423] Antibody (SR1995)
Immunohistochemistry: Smad3 [p Ser 423] Antibody (SR1995) [NBP3-21516] -
Immunohistochemistry: Smad3 [p Ser 423] Antibody (SR1995) [NBP3-21516] - Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma, using NBP3-21516.Applications for Smad3 [p Ser423] Antibody (SR1995)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl, 50% glycerol.
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Smad3
Long Name
Mothers Against DPP Homolog 3
Alternate Names
DKFZp586N0721, DKFZp686J10186, hMAD-3, hSMAD3, HsT17436, JV15-2MGC60396, MAD homolog 3, mad protein homolog, MAD, mothers against decapentaplegic homolog 3, MAD, mothers against decapentaplegic homolog 3 (Drosophila), Mad3, MADH3mad homolog JV15-2, mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMA- and MAD-related protein 3, SMAD 3, SMAD family member 3HSPC193, SMAD, mothers against DPP homolog 3, SMAD, mothers against DPP homolog 3 (Drosophila), Smad3
Gene Symbol
SMAD3
Additional Smad3 Products
Product Documents for Smad3 [p Ser423] Antibody (SR1995)
Product Specific Notices for Smad3 [p Ser423] Antibody (SR1995)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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