Smad4 Antibody (SMAD4/2440)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07262

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # SMAD4/2440

Concentration

0.2 mg/ml

View all Smad4 Antibodies »

Product Specifications

Immunogen

Recombinant full-length human Smad4 protein (Uniprot: Q13485 )

Localization

Cytoplasmic & Nuclear

Marker

Pancreatic Adenocarcinoma Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08320)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Smad4 Antibody (SMAD4/2440)

Immunohistochemistry-Paraffin: Smad4 Antibody (SMAD4/2440) [NBP3-07262]

Immunohistochemistry-Paraffin: Smad4 Antibody (SMAD4/2440) [NBP3-07262]

Immunohistochemistry-Paraffin: Smad4 Antibody (SMAD4/2440) [NBP3-07262] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Smad4 Mouse Monoclonal Antibody (Smad4/2440).
Protein Array: Smad4 Antibody (SMAD4/2440) [NBP3-07262]

Protein Array: Smad4 Antibody (SMAD4/2440) [NBP3-07262]

Protein Array: Smad4 Antibody (SMAD4/2440) [NBP3-07262] - Analysis of Protein Array containing >19,000 full-length human proteins using Smad4 Mouse Monoclonal Antibody (Smad4/2440).

Applications for Smad4 Antibody (SMAD4/2440)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Smad4

Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFbeta/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MadH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFbeta and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFbeta signaling by interfering with TGFbeta-mediated phosphorylation of other Smad family members.

Long Name

Mothers Against DPP Homolog 4

Alternate Names

DPC4, MADH4

Gene Symbol

SMAD4

UniProt

Q13485

Additional Smad4 Products

Product Documents for Smad4 Antibody (SMAD4/2440)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Smad4 Antibody (SMAD4/2440)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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