Smad5 Antibody (3H9) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37648

Novus Biologicals, part of Bio-Techne
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NBP2-37648
NBP2-37648-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3H9

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Smad5 Antibodies »

Product Summary for Smad5 Antibody (3H9) - BSA Free

Immunogen

Purified recombinant fragment of human SMAD5 expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Smad5 Antibody (3H9) - BSA Free

Western Blot: Smad5 Antibody (3H9)BSA Free [NBP2-37648]

Western Blot: Smad5 Antibody (3H9)BSA Free [NBP2-37648]

Western Blot: Smad5 Antibody (3H9) [NBP2-37648] - Analysis using SMAD5 mouse mAb against Hela (1), SK-N-SH (2), PC-12 (3), Jurkat (4), and K562 (5) cell lysate.
Immunocytochemistry/ Immunofluorescence: Smad5 Antibody (3H9) - BSA Free [NBP2-37648]

Immunocytochemistry/ Immunofluorescence: Smad5 Antibody (3H9) - BSA Free [NBP2-37648]

Immunocytochemistry/Immunofluorescence: Smad5 Antibody (3H9) [NBP2-37648] - Analysis of NTERA-2 cells using SMAD5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: Smad5 Antibody (3H9) - BSA Free [NBP2-37648]

Immunohistochemistry-Paraffin: Smad5 Antibody (3H9) - BSA Free [NBP2-37648]

Immunohistochemistry-Paraffin: Smad5 Antibody (3H9) [NBP2-37648] - Analysis of tissues (left) and lung cancer tissues (right) using SMAD5 mouse mAb with DAB staining.
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Applications for Smad5 Antibody (3H9) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Smad5

SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily (1). SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, SMAD2, SMAD5, SMAD8 and SMAD9), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and SMAD7). SMAD1, SMAD5, SMAD8 and SMAD9 have high degrees of homology and antibodies are available that recognize sequences common to all of them. SMAD8 and SMAD9 are typically used as alternate names for one another in the literature. Human SMAD1 is a 465 amino acid protein; GenBank Accession No. AAP36050.1. Human SMAD2 is a 467 amino acid protein; GenBank Accession No. AAC51918.1 Human SMAD3 is a 425 amino acid protein; GenBank Accession No. NP_005893.1 Human SMAD4 is a 552 amino acid protein GenBank Accession No. NP_005350.1. Human SMAD5 is a 465 amino acid protein; GenBank Accession No. AAC50791.1. Human SMAD6 is a 496 amino acid protein; GenBank Accession No. AAH12986.1 Human SMAD7 is a 426 amino acid protein; GenBank Accession No. AAB81354.1 Mouse SMAD8 is a 430 amino acid protein; GenBank Accession No. AAN85445.1 Human SMAD9 is a 430 amino acid protein; GenBank Accession No. NP_005896.1.

Long Name

Mothers Against DPP Homolog 5

Alternate Names

Dwfc, JV5-1, MADH5

Gene Symbol

SMAD5

Additional Smad5 Products

Product Documents for Smad5 Antibody (3H9) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Smad5 Antibody (3H9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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