Smad7 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87728
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Predicted:
Mouse (99%), Rat (99%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Cited:
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: EVKRLCCCESYGKINPELVCCNPHHLSRLCELESPPPPYSRYPMDFLKPTADCPDAVPSSAETGGTNYLAPGGLSDSQLLLEPGDRSHWCVVAYWEEKTRVGRLYCVQEPSLDIFYDLPQGNG
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Smad7 Antibody
Immunocytochemistry/ Immunofluorescence: Smad7 Antibody [NBP1-87728]
Immunocytochemistry/Immunofluorescence: Smad7 Antibody [NBP1-87728] - Staining of human cell line U-251 MG shows localization to nucleoplasm, nucleoli fibrillar center, cytosol and centrosome. Antibody staining is shown in green.Immunohistochemistry-Paraffin: Smad7 Antibody [NBP1-87728]
Immunohistochemistry-Paraffin: Smad7 Antibody [NBP1-87728] - Staining of human Liver shows very weak cytoplasmic positivity in hepatocytes.Immunohistochemistry-Paraffin: Smad7 Antibody [NBP1-87728]
Immunohistochemistry-Paraffin: Smad7 Antibody [NBP1-87728] - Staining of human lung shows nuclear positivity in pneumnocytes and macrophages.Applications for Smad7 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50-1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Smad7
Long Name
Mothers Against DPP Homolog 7
Alternate Names
MADH7, MADH8
Gene Symbol
SMAD7
Additional Smad7 Products
Product Documents for Smad7 Antibody
Product Specific Notices for Smad7 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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