Smad7 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24710
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Sheep
Cited:
Human, Mouse, Ovine
Predicted:
Rat (90%). Backed by our 100% Guarantee.
Applications
Validated:
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
ELISA, IF/IHC, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
This antibody was raised against two peptides made from within amino acids 10 and 50 of human SMAD7. This sequence is 100% or highly conserved between human and multiple species.
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 24576683).
Specificity
This antibody may cross-react with SMAD6 depending on cell or tissue-specific expression.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Smad7 Antibody
Western Blot: Smad7 Antibody [NBP2-24710]
Western Blot: Smad7 Antibody [NBP2-24710] - Effect of mTOR inhibitors on TGF-beta activation of mTOR and Smad pathways. Serum-deprived IPF fibroblasts were treated with or without TGF-beta for 15 minutes in the presence or absence of indicated inhibitors followed by Western blot analysis with an anti-phospho-Smad2 or Smad3 antibody. Expression of total Smad-2, 3, 4 and 7 was analyzed by Western blot. Experiment was done on three lines, which are shown in Figure 1; results were similar between the three lines and results from the IPF fibroblasts isolated from surgical lung biopsy are shown here. Image collected and cropped by CiteAb from the following publication (https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0106155), licensed under a CC-BY license.Immunohistochemistry-Paraffin: Smad7 Antibody [NBP2-24710]
Immunohistochemistry-Paraffin: Smad7 Antibody [NBP2-24710] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human liver using Smad7 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining of Smad7 was observed. Staining was performed by Histowiz.Western Blot: Smad7 Antibody [NBP2-24710]
Western Blot: Smad7 Antibody [NBP2-24710] - Analysis using SMAD7 antibody. Lysate from HepG2 cells probed with SMAD7 antibody.Applications for Smad7 Antibody
Application
Recommended Usage
ELISA
reported in scientific literature (PMID 32108968)
Immunohistochemistry
5-10 ug/ml
Immunohistochemistry-Paraffin
5 ug/ml
Western Blot
2-5 ug/ml
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Smad7
Long Name
Mothers Against DPP Homolog 7
Alternate Names
MADH7, MADH8
Gene Symbol
SMAD7
UniProt
Additional Smad7 Products
Product Documents for Smad7 Antibody
Product Specific Notices for Smad7 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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