Smallpox B5R Antibody (1J2F3) [CoraFluor™ 1]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24699CL1

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Virus

Applications

Western Blot

Label

CoraFluor 1

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1J2F3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Smallpox B5R Antibodies »

Product Specifications

Immunogen

Amino acids 33-47 (IQEYNLIRTYKKVDK) ofthe B5R protein were used as the immunogen (NCBI Reference Sequence: NP_042218.1)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for Smallpox B5R Antibody (1J2F3) [CoraFluor™ 1]

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: Smallpox B5R

Vaccinia virus, a member of Poxviridae family, is a dsDNA enveloped virus. It consists of 4 types of virion stages among which extracellular enveloped virus (EEV) is critical for cell-to-cell and long range viral infection. B5R is an EEV-specific Type I membrane glycoprotein essential for EEV formation. It consists of a large ectodomain containing regions similar to SCR domains of complement regulatory proteins followed by a transmembrane region and a cytoplasmic tail. B5R is essential for efficient wrapping of IMV, actin tail formation, normal plaque size, virus virulence, incorporation of the protein into EEV and for EEV formation.

Alternate Names

B5R protein, B6R, B7R, B7R protein, EEV membrane glycoprotein, Glycoprotein, H7R, H7R protein, ORF5R, VARVgp175

Gene Symbol

B7R

Additional Smallpox B5R Products

Product Documents for Smallpox B5R Antibody (1J2F3) [CoraFluor™ 1]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Smallpox B5R Antibody (1J2F3) [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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