Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all SMARCA1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1004 and 1054 of human SMARCA1/SNF2L using the numbering given in entry NP_003060.2 (GeneID 6594).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SMARCA1 Antibody

Immunohistochemistry: SMARCA1 Antibody [NB100-57524]

Immunohistochemistry: SMARCA1 Antibody [NB100-57524]

Immunohistochemistry: SMARCA1 Antibody [NB100-57524] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- SMARCA1/SNF2L used at a dilution of 1:1,000 (1 ug/ml). Detection: DAB
Immunoprecipitation: SMARCA1 Antibody [NB100-57524]

Immunoprecipitation: SMARCA1 Antibody [NB100-57524]

Immunoprecipitation: SMARCA1 Antibody [NB100-57524] - Detection of human SMARCA1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMARCA1 antibody NB100-57524 (lot 2) used for IP at 6 ug per reaction. SMARCA1 was also immunoprecipitated by a previous lot of this antibody (lot 1). For blotting immunoprecipitated SMARCA1, NB100-57524 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for SMARCA1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:5000

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

2-5 mcg/mg

Western Blot

1:100-1:2000
Application Notes
This antibody is not recommended for blotting crude sources of SMARCA1/SNF2L. NB100-57524 can be used at 1:2,000 to 1:10,000 for blotting enriched sources (e.g. immunoprecipitated and exogenously expressed) of SMARCA1/SNF2L. Immunohistochemistry 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SMARCA1

The SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin (SMARC), also called BRG1-associated factors (BAFs), have been identified as components of the human SWI/SNF-like chromatin-remodeling protein complexes. Member of this family have helicase and ATPase activities that alter chromatin structure to facilitate gene transcription. SMARCA1/SNF2L is a component of the human NURF (nucleosome remodeling factor) involved in the regulation of genes involved in neuronal development. SMARCA1/SNF2L is also a component of the heterodimeric complex CERF [CECR2(cat eye syndrome chromosome region, candidate 2)-containing remodeling factor]. Alternate names for SMARCA1/SNF2L include SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a1, SNF2, SNF2L, SNF2L1, SNF2LB, sucrose nonfermenting 2-like protein 1, NURF140, and ISWI.

Alternate Names

ATP-dependent helicase SMARCA1, DKFZp686D1623, EC 3.6.1, EC 3.6.4.-, FLJ41547, global transcription activator homologous sequence, Nucleosome-remodeling factor subunit SNF2L, NURF140, probable global transcription activator SNF2L1, SNF2L, SNF2L1ISWI, SNF2LB, SNF2-like 1, SNF2LT, subfamily a, member 1, sucrose nonfermenting 2-like protein 1, SWI, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a1, SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A member 1, SWI2

Entrez Gene IDs

6594 (Human)

Gene Symbol

SMARCA1

UniProt

NP_003060.2 (Human)

Additional SMARCA1 Products

Product Documents for SMARCA1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SMARCA1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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