SMC1 Antibody (5B6) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51596

Novus Biologicals, part of Bio-Techne
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NBP1-51596
NBP1-51596-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5B6

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SMC1 Antibodies »

Product Summary for SMC1 Antibody (5B6) - BSA Free

Immunogen

Purified recombinant fragment of human SMC1 expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

143 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SMC1 Antibody (5B6) - BSA Free

Western Blot: SMC1 Antibody (5B6)BSA Free [NBP1-51596]

Western Blot: SMC1 Antibody (5B6)BSA Free [NBP1-51596]

Western Blot: SMC1 Antibody (5B6) [NBP1-51596] - Analysis using SMC1 mouse mAb against K562 (1), Jurkat (2) and A549 (3) cell lysate.
Immunocytochemistry/ Immunofluorescence: SMC1 Antibody (5B6) - BSA Free [NBP1-51596]

Immunocytochemistry/ Immunofluorescence: SMC1 Antibody (5B6) - BSA Free [NBP1-51596]

Immunocytochemistry/Immunofluorescence: SMC1 Antibody (5B6) [NBP1-51596] - Analysis of NIH3T3 cells using SMC1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: SMC1 Antibody (5B6) - BSA Free [NBP1-51596]

Immunohistochemistry-Paraffin: SMC1 Antibody (5B6) - BSA Free [NBP1-51596]

Immunohistochemistry-Paraffin: SMC1 Antibody (5B6) [NBP1-51596] - Analysis of paraffin-embedded human colon using SMC1 mouse mAb with DAB staining.
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Applications for SMC1 Antibody (5B6) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SMC1

SMC1L1/SMC1 (structural maintenance of chromosome 1-like 1 (yeast)) is a 145-160 kD heterodimeric protein that is a member of the SMC family. This nuclear protein dissociates from chromosomes before mitosis but remains associated with kinetochore during mitosis. SMC1L1/SMC1 is located on the X-chromosome and is involved in mitotic chromosome dynamics. The SMC family includes SMC3, SMC1L1, and SMC1L2 which form part of the cohesion multiprotein complex. This protein can also form heterodimers consisting of SMC1L1/SMC3 or SMCL2/SMC3 and may also be involved in DNA repair. SMC1L1/SMC1 is phosphorylated by ATM after DNA damage; phosphorylation is also required for sister chromatid cohesion and correct segregation of chromosomes during cell division. SMC1L1/SMC1 associates with BRCA1, and SYCP2, and interacts directly with SMC3 in the cohesion complex (linked by RAD21 complexed to one STAG protein). The Poly6096 antibody recognizes the N-terminal region of human and mouse SMC1L1/SMC1 and has been shown to be useful for Western blotting and immunofluorescence staining. BRCA1, SYCP2, interacts directly with SMC3 in the cohesion complex (linked by RAD21 complexed to one STAG protein)

Long Name

Structural Maintenance of Chromosomes 1

Alternate Names

SB1.8, SMC1A, SMC1L1

Gene Symbol

SMC1A

Additional SMC1 Products

Product Documents for SMC1 Antibody (5B6) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SMC1 Antibody (5B6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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