SMC1 Antibody (8C11)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26313
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 8C11
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for SMC1 Antibody (8C11)
Immunogen
A synthesized peptide derived from Human SMC1 [UniProt Q14683]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SMC1 Antibody (8C11)
Immunohistochemistry: SMC1 Antibody (8C11) [NBP3-26313] -
Immunohistochemistry: SMC1 Antibody (8C11) [NBP3-26313] - Image of SMC1 Antibody (8C11) diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunohistochemistry: SMC1 Antibody (8C11) [NBP3-26313] -
Immunohistochemistry: SMC1 Antibody (8C11) [NBP3-26313] - Image of SMC1 Antibody (8C11) diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Western Blot: SMC1 Antibody (8C11) [NBP3-26313] -
Western Blot: SMC1 Antibody (8C11) [NBP3-26313] - Positive Western Blot detected in: Hela whole cell lysate, K562 whole cell lysate, 293T whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate, HL60 whole cell lysate, MCF-7 whole cell lysate.All lanes: SMC1 Antibody at 1: 1500
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 144 kDa
Observed band size: 144 kDa
Applications for SMC1 Antibody (8C11)
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: SMC1
Long Name
Structural Maintenance of Chromosomes 1
Alternate Names
SB1.8, SMC1A, SMC1L1
Gene Symbol
SMC1A
Additional SMC1 Products
Product Documents for SMC1 Antibody (8C11)
Product Specific Notices for SMC1 Antibody (8C11)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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