SMC1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-204
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Predicted:
Bovine (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot
Cited:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 1175 and the C-terminus of human Structural Maintenance of Chromosomes 1 using the numbering given in entry NP_006297.2
Localization
Nuclear
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
160 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB100-322, NB100-224, NB100-206
Scientific Data Images for SMC1 Antibody
Immunohistochemistry-Paraffin: SMC1 Antibody [NB100-204]
Immunohistochemistry-Paraffin: SMC1 Antibody [NB100-204] - FFPE section of mouse colon carcinoma CT26. Antibody: Affinity purified rabbit anti-SMC1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DABImmunohistochemistry-Paraffin: SMC1 Antibody [NB100-204]
Immunohistochemistry-Paraffin: SMC1 Antibody [NB100-204] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-SMC1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DABSimple Western: SMC1 Antibody [NB100-204]
Simple Western: SMC1 Antibody [NB100-204] - Simple Western lane view shows a specific band for SMC1 in 0.5 mg/ml of Hek293 (left) and HeLa (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.Applications for SMC1 Antibody
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
1:250- 1:1000
Immunohistochemistry
1:2000 -1:10000
Immunohistochemistry-Paraffin
1:2000 -1:10000
Immunoprecipitation
2 - 10 ug/mg lysate
Simple Western
1:100
Western Blot
1:2000- 1:10000
Application Notes
ELISA and Immunoprecipitation were reported in (PMID: 19409520).
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: SMC1
Long Name
Structural Maintenance of Chromosomes 1
Alternate Names
SB1.8, SMC1A, SMC1L1
Gene Symbol
SMC1A
Additional SMC1 Products
Product Documents for SMC1 Antibody
Product Specific Notices for SMC1 Antibody
This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB100-322, NB100-224, NB100-206
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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