SMC2 Antibody (PSH0-63)

Western Blot: SMC2 Antibody (PSH0-63) [NBP3-32959]

Western Blot: SMC2 Antibody (PSH0-63) [NBP3-32959] - Western blot analysis of SMC2 on different lysates with Rabbit anti-SMC2 antibody (NBP3-32959) at 1/1,000 dilution.

Lane 1: HeLa cell lysate (30 ug/Lane)
Lane 2: HEK-293 cell lysate (30 ug/Lane)
Lane 3: HT-29 cell lysate (30 ug/Lane)
Lane 4: HepG2 cell lysate (30 ug/Lane)
Lane 5: A431 cell lysate (30 ug/Lane)
Lane 6: SH-SY5Y cell lysate (30 ug/Lane)
Lane 7: Jurkat cell lysate (30 ug/Lane)
Lane 8: Raji cell lysate (30 ug/Lane)
Lane 9: COS-1 cell lysate (24 ug/Lane)

Predicted band size: 136 kDa
Observed band size: 136 kDa

Exposure time: 1 minute;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32959) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: SMC2 Antibody (PSH0-63) [NBP3-32959]

Immunohistochemistry: SMC2 Antibody (PSH0-63) [NBP3-32959] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-SMC2 antibody (NBP3-32959) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32959) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: SMC2 Antibody (PSH0-63) [NBP3-32959]

Immunocytochemistry/ Immunofluorescence: SMC2 Antibody (PSH0-63) [NBP3-32959] - Immunocytochemistry analysis of HeLa cells labeling SMC2 with Rabbit anti-SMC2 antibody (NBP3-32959) at 1/100 dilution.

Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SMC2 antibody (NBP3-32959) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.