SMC3 Antibody (2F11)

Novus Biologicals, part of Bio-Techne | Catalog # H00009126-M02

Novus Biologicals, part of Bio-Techne
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H00009126-M02
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 2F11

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SMC3 Antibodies »

Product Specifications

Immunogen

CSPG6 (AAH47324, 711 a.a. ~ 810 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.IDQLMNQMQQIETQQRKFKASRDSILSEMKMLKEKRQQSEKTFMPKQRSLQSLEASLHAMESTRESLKAELGTDLLSQLSLEDQKRVDALNDEIRQLQQE

Specificity

CSPG6 - chondroitin sulfate proteoglycan 6 (bamacan) (2F11)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for SMC3 Antibody (2F11)

Western Blot: SMC3 Antibody (2F11) [H00009126-M02]

Western Blot: SMC3 Antibody (2F11) [H00009126-M02]

Western Blot: SMC3 Antibody (2F11) [H00009126-M02] - Analysis of SMC3 expression in transfected 293T cell line by CSPG6 monoclonal antibody (M02), clone 2F11.Lane 1: SMC3 transfected lysate(141.5 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: SMC3 Antibody (2F11) [H00009126-M02] - Detection limit for recombinant GST tagged CSPG6 is approximately 0.3ng/ml as a capture antibody.

Applications for SMC3 Antibody (2F11)

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1:500
Application Notes
Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SMC3

This gene belongs to the SMC3 subfamily of SMC proteins. The encoded protein occurs in certain cell types as either an intracellular, nuclear protein or a secreted protein. The nuclear form, known as structural maintenance of chromosomes 3, is a component of the multimeric cohesin complex that holds together sister chromatids during mitosis, enabling proper chromosome segregation. Post-translational modification of the encoded protein by the addition of chondroitin sulfate chains gives rise to the secreted proteoglycan bamacan, an abundant basement membrane protein. [provided by RefSeq]

Alternate Names

Bamacan, BAMSMC protein 3, Basement membrane-associated chondroitin proteoglycan, BMH, Chondroitin sulfate proteoglycan 6, chondroitin sulfate proteoglycan 6 (bamacan), Chromosome-associated polypeptide, CSPG6structural maintenance of chromosomes protein 3, hCAP, HCAPbamacan, SMC-3, SMC3L1CDLS3, structural maintenance of chromosomes 3

Entrez Gene IDs

9126 (Human)

Gene Symbol

SMC3

UniProt

AAH47324 (Human)

Additional SMC3 Products

Product Documents for SMC3 Antibody (2F11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SMC3 Antibody (2F11)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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